Cargando…
Functional Dissection of the Neural Substrates for Sexual Behaviors in Drosophila melanogaster
The male-specific Fruitless proteins (Fru(M)) act to establish the potential for male courtship behavior in Drosophila melanogaster and are expressed in small groups of neurons throughout the nervous system. We screened ∼1000 GAL4 lines, using assays for general courtship, male–male interactions, an...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Genetics Society of America
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3176112/ https://www.ncbi.nlm.nih.gov/pubmed/21705753 http://dx.doi.org/10.1534/genetics.111.129940 |
_version_ | 1782212178973032448 |
---|---|
author | Meissner, Geoffrey W. Manoli, Devanand S. Chavez, Jose F. Knapp, Jon-Michael Lin, Tasha L. Stevens, Robin J. Mellert, David J. Tran, David H. Baker, Bruce S. |
author_facet | Meissner, Geoffrey W. Manoli, Devanand S. Chavez, Jose F. Knapp, Jon-Michael Lin, Tasha L. Stevens, Robin J. Mellert, David J. Tran, David H. Baker, Bruce S. |
author_sort | Meissner, Geoffrey W. |
collection | PubMed |
description | The male-specific Fruitless proteins (Fru(M)) act to establish the potential for male courtship behavior in Drosophila melanogaster and are expressed in small groups of neurons throughout the nervous system. We screened ∼1000 GAL4 lines, using assays for general courtship, male–male interactions, and male fertility to determine the phenotypes resulting from the GAL4-driven inhibition of Fru(M) expression in subsets of these neurons. A battery of secondary assays showed that the phenotypic classes of GAL4 lines could be divided into subgroups on the basis of additional neurobiological and behavioral criteria. For example, in some lines, restoration of Fru(M) expression in cholinergic neurons restores fertility or reduces male–male courtship. Persistent chains of males courting each other in some lines results from males courting both sexes indiscriminately, whereas in other lines this phenotype results from apparent habituation deficits. Inhibition of ectopic Fru(M) expression in females, in populations of neurons where Fru(M) is necessary for male fertility, can rescue female infertility. To identify the neurons responsible for some of the observed behavioral alterations, we determined the overlap between the identified GAL4 lines and endogenous Fru(M) expression in lines with fertility defects. The GAL4 lines causing fertility defects generally had widespread overlap with Fru(M) expression in many regions of the nervous system, suggesting likely redundant Fru(M)-expressing neuronal pathways capable of conferring male fertility. From associations between the screened behaviors, we propose a functional model for courtship initiation. |
format | Online Article Text |
id | pubmed-3176112 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Genetics Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-31761122011-10-19 Functional Dissection of the Neural Substrates for Sexual Behaviors in Drosophila melanogaster Meissner, Geoffrey W. Manoli, Devanand S. Chavez, Jose F. Knapp, Jon-Michael Lin, Tasha L. Stevens, Robin J. Mellert, David J. Tran, David H. Baker, Bruce S. Genetics Investigations The male-specific Fruitless proteins (Fru(M)) act to establish the potential for male courtship behavior in Drosophila melanogaster and are expressed in small groups of neurons throughout the nervous system. We screened ∼1000 GAL4 lines, using assays for general courtship, male–male interactions, and male fertility to determine the phenotypes resulting from the GAL4-driven inhibition of Fru(M) expression in subsets of these neurons. A battery of secondary assays showed that the phenotypic classes of GAL4 lines could be divided into subgroups on the basis of additional neurobiological and behavioral criteria. For example, in some lines, restoration of Fru(M) expression in cholinergic neurons restores fertility or reduces male–male courtship. Persistent chains of males courting each other in some lines results from males courting both sexes indiscriminately, whereas in other lines this phenotype results from apparent habituation deficits. Inhibition of ectopic Fru(M) expression in females, in populations of neurons where Fru(M) is necessary for male fertility, can rescue female infertility. To identify the neurons responsible for some of the observed behavioral alterations, we determined the overlap between the identified GAL4 lines and endogenous Fru(M) expression in lines with fertility defects. The GAL4 lines causing fertility defects generally had widespread overlap with Fru(M) expression in many regions of the nervous system, suggesting likely redundant Fru(M)-expressing neuronal pathways capable of conferring male fertility. From associations between the screened behaviors, we propose a functional model for courtship initiation. Genetics Society of America 2011-09 /pmc/articles/PMC3176112/ /pubmed/21705753 http://dx.doi.org/10.1534/genetics.111.129940 Text en Copyright © 2011 by the Genetics Society of America Available freely online through the author-supported open access option. |
spellingShingle | Investigations Meissner, Geoffrey W. Manoli, Devanand S. Chavez, Jose F. Knapp, Jon-Michael Lin, Tasha L. Stevens, Robin J. Mellert, David J. Tran, David H. Baker, Bruce S. Functional Dissection of the Neural Substrates for Sexual Behaviors in Drosophila melanogaster |
title | Functional Dissection of the Neural Substrates for Sexual Behaviors in Drosophila melanogaster |
title_full | Functional Dissection of the Neural Substrates for Sexual Behaviors in Drosophila melanogaster |
title_fullStr | Functional Dissection of the Neural Substrates for Sexual Behaviors in Drosophila melanogaster |
title_full_unstemmed | Functional Dissection of the Neural Substrates for Sexual Behaviors in Drosophila melanogaster |
title_short | Functional Dissection of the Neural Substrates for Sexual Behaviors in Drosophila melanogaster |
title_sort | functional dissection of the neural substrates for sexual behaviors in drosophila melanogaster |
topic | Investigations |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3176112/ https://www.ncbi.nlm.nih.gov/pubmed/21705753 http://dx.doi.org/10.1534/genetics.111.129940 |
work_keys_str_mv | AT meissnergeoffreyw functionaldissectionoftheneuralsubstratesforsexualbehaviorsindrosophilamelanogaster AT manolidevanands functionaldissectionoftheneuralsubstratesforsexualbehaviorsindrosophilamelanogaster AT chavezjosef functionaldissectionoftheneuralsubstratesforsexualbehaviorsindrosophilamelanogaster AT knappjonmichael functionaldissectionoftheneuralsubstratesforsexualbehaviorsindrosophilamelanogaster AT lintashal functionaldissectionoftheneuralsubstratesforsexualbehaviorsindrosophilamelanogaster AT stevensrobinj functionaldissectionoftheneuralsubstratesforsexualbehaviorsindrosophilamelanogaster AT mellertdavidj functionaldissectionoftheneuralsubstratesforsexualbehaviorsindrosophilamelanogaster AT trandavidh functionaldissectionoftheneuralsubstratesforsexualbehaviorsindrosophilamelanogaster AT bakerbruces functionaldissectionoftheneuralsubstratesforsexualbehaviorsindrosophilamelanogaster |