Phylogenetic and Pathotypical Analysis of Two Virulent Newcastle Disease Viruses Isolated from Domestic Ducks in China

Two velogenic Newcastle disease viruses (NDV) obtained from outbreaks in domestic ducks in China were characterized in this study. Phylogenetic analysis revealed that both strains clustered with the class II viruses, with one phylogenetically close to the genotype VII NDVs and the other closer to ge...

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Autores principales: Zhang, Shouping, Wang, Xiaoting, Zhao, Changguang, Liu, Dehua, Hu, Yanxin, Zhao, Jixun, Zhang, Guozhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3176290/
https://www.ncbi.nlm.nih.gov/pubmed/21949828
http://dx.doi.org/10.1371/journal.pone.0025000
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author Zhang, Shouping
Wang, Xiaoting
Zhao, Changguang
Liu, Dehua
Hu, Yanxin
Zhao, Jixun
Zhang, Guozhong
author_facet Zhang, Shouping
Wang, Xiaoting
Zhao, Changguang
Liu, Dehua
Hu, Yanxin
Zhao, Jixun
Zhang, Guozhong
author_sort Zhang, Shouping
collection PubMed
description Two velogenic Newcastle disease viruses (NDV) obtained from outbreaks in domestic ducks in China were characterized in this study. Phylogenetic analysis revealed that both strains clustered with the class II viruses, with one phylogenetically close to the genotype VII NDVs and the other closer to genotype IX. The deduced amino acid sequence of the cleavage site of the fusion (F) protein confirmed that both isolates contained the virulent motif (112)RRQK/RRF(117) at the cleavage site. The two NDVs had severe pathogenicity in fully susceptible chickens, resulting in 100% mortality. One of the isolates also demonstrated some pathogenicity in domestic ducks. The present study suggests that more than one genotype of NDV circulates in domestic ducks in China and viral transmission may occur among chickens and domestic ducks.
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spelling pubmed-31762902011-09-26 Phylogenetic and Pathotypical Analysis of Two Virulent Newcastle Disease Viruses Isolated from Domestic Ducks in China Zhang, Shouping Wang, Xiaoting Zhao, Changguang Liu, Dehua Hu, Yanxin Zhao, Jixun Zhang, Guozhong PLoS One Research Article Two velogenic Newcastle disease viruses (NDV) obtained from outbreaks in domestic ducks in China were characterized in this study. Phylogenetic analysis revealed that both strains clustered with the class II viruses, with one phylogenetically close to the genotype VII NDVs and the other closer to genotype IX. The deduced amino acid sequence of the cleavage site of the fusion (F) protein confirmed that both isolates contained the virulent motif (112)RRQK/RRF(117) at the cleavage site. The two NDVs had severe pathogenicity in fully susceptible chickens, resulting in 100% mortality. One of the isolates also demonstrated some pathogenicity in domestic ducks. The present study suggests that more than one genotype of NDV circulates in domestic ducks in China and viral transmission may occur among chickens and domestic ducks. Public Library of Science 2011-09-19 /pmc/articles/PMC3176290/ /pubmed/21949828 http://dx.doi.org/10.1371/journal.pone.0025000 Text en Zhang et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhang, Shouping
Wang, Xiaoting
Zhao, Changguang
Liu, Dehua
Hu, Yanxin
Zhao, Jixun
Zhang, Guozhong
Phylogenetic and Pathotypical Analysis of Two Virulent Newcastle Disease Viruses Isolated from Domestic Ducks in China
title Phylogenetic and Pathotypical Analysis of Two Virulent Newcastle Disease Viruses Isolated from Domestic Ducks in China
title_full Phylogenetic and Pathotypical Analysis of Two Virulent Newcastle Disease Viruses Isolated from Domestic Ducks in China
title_fullStr Phylogenetic and Pathotypical Analysis of Two Virulent Newcastle Disease Viruses Isolated from Domestic Ducks in China
title_full_unstemmed Phylogenetic and Pathotypical Analysis of Two Virulent Newcastle Disease Viruses Isolated from Domestic Ducks in China
title_short Phylogenetic and Pathotypical Analysis of Two Virulent Newcastle Disease Viruses Isolated from Domestic Ducks in China
title_sort phylogenetic and pathotypical analysis of two virulent newcastle disease viruses isolated from domestic ducks in china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3176290/
https://www.ncbi.nlm.nih.gov/pubmed/21949828
http://dx.doi.org/10.1371/journal.pone.0025000
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