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A Left/Right Asymmetric Neuronal Differentiation Program Is Controlled by the Caenorhabditis elegans LSY-27 Zinc-Finger Transcription Factor

Functional diversification across the left/right axis is a common feature of many nervous systems. The genetic programs that control left/right asymmetric neuron function and gene expression in the nervous system are, however, poorly understood. We describe here the molecular characterization of two...

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Detalles Bibliográficos
Autores principales: Zhang, Feifan, O'Meara, M. Maggie, Hobert, Oliver
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3176537/
https://www.ncbi.nlm.nih.gov/pubmed/21555395
http://dx.doi.org/10.1534/genetics.111.129064
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author Zhang, Feifan
O'Meara, M. Maggie
Hobert, Oliver
author_facet Zhang, Feifan
O'Meara, M. Maggie
Hobert, Oliver
author_sort Zhang, Feifan
collection PubMed
description Functional diversification across the left/right axis is a common feature of many nervous systems. The genetic programs that control left/right asymmetric neuron function and gene expression in the nervous system are, however, poorly understood. We describe here the molecular characterization of two phenotypically similar mutant Caenorhabditis elegans strains in which left/right asymmetric gene expression programs of two gustatory neurons, called ASEL and ASER, are disrupted such that the differentiation program of the ASER neuron is derepressed in the ASEL neuron. We show that in one mutant strain the LIM homeobox gene lim-6 is defective whereas in another strain a novel member of a nematode-specific, fast-evolving family of C2H2 zinc-finger transcription factors, lsy-27, is mutated, as revealed by whole-genome sequencing. lsy-27 is broadly and exclusively expressed in the embryo and acts during the initiation, but not during the maintenance phase of ASE asymmetry control to assist in the initiation of lim-6 expression.
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spelling pubmed-31765372011-09-20 A Left/Right Asymmetric Neuronal Differentiation Program Is Controlled by the Caenorhabditis elegans LSY-27 Zinc-Finger Transcription Factor Zhang, Feifan O'Meara, M. Maggie Hobert, Oliver Genetics Note Functional diversification across the left/right axis is a common feature of many nervous systems. The genetic programs that control left/right asymmetric neuron function and gene expression in the nervous system are, however, poorly understood. We describe here the molecular characterization of two phenotypically similar mutant Caenorhabditis elegans strains in which left/right asymmetric gene expression programs of two gustatory neurons, called ASEL and ASER, are disrupted such that the differentiation program of the ASER neuron is derepressed in the ASEL neuron. We show that in one mutant strain the LIM homeobox gene lim-6 is defective whereas in another strain a novel member of a nematode-specific, fast-evolving family of C2H2 zinc-finger transcription factors, lsy-27, is mutated, as revealed by whole-genome sequencing. lsy-27 is broadly and exclusively expressed in the embryo and acts during the initiation, but not during the maintenance phase of ASE asymmetry control to assist in the initiation of lim-6 expression. Genetics Society of America 2011-07 /pmc/articles/PMC3176537/ /pubmed/21555395 http://dx.doi.org/10.1534/genetics.111.129064 Text en Copyright © 2011 by the Genetics Society of America Available freely online through the author-supported open access option.
spellingShingle Note
Zhang, Feifan
O'Meara, M. Maggie
Hobert, Oliver
A Left/Right Asymmetric Neuronal Differentiation Program Is Controlled by the Caenorhabditis elegans LSY-27 Zinc-Finger Transcription Factor
title A Left/Right Asymmetric Neuronal Differentiation Program Is Controlled by the Caenorhabditis elegans LSY-27 Zinc-Finger Transcription Factor
title_full A Left/Right Asymmetric Neuronal Differentiation Program Is Controlled by the Caenorhabditis elegans LSY-27 Zinc-Finger Transcription Factor
title_fullStr A Left/Right Asymmetric Neuronal Differentiation Program Is Controlled by the Caenorhabditis elegans LSY-27 Zinc-Finger Transcription Factor
title_full_unstemmed A Left/Right Asymmetric Neuronal Differentiation Program Is Controlled by the Caenorhabditis elegans LSY-27 Zinc-Finger Transcription Factor
title_short A Left/Right Asymmetric Neuronal Differentiation Program Is Controlled by the Caenorhabditis elegans LSY-27 Zinc-Finger Transcription Factor
title_sort left/right asymmetric neuronal differentiation program is controlled by the caenorhabditis elegans lsy-27 zinc-finger transcription factor
topic Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3176537/
https://www.ncbi.nlm.nih.gov/pubmed/21555395
http://dx.doi.org/10.1534/genetics.111.129064
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