An Alternative Paper Based Tissue Washing Method for Mass Spectrometry Imaging: Localized Washing and Fragile Tissue Analysis

Surface treatment of biological tissue sections improves detection of peptides and proteins for mass spectrometry imaging. However, liquid surface treatments can result in diffusion of surface analytes and fragile tissue sections can be easily damaged by typical washing solvents. Here, we present a...

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Autores principales: Amstalden van Hove, Erika R., Smith, Donald F., Fornai, Lara, Glunde, Kristine, Heeren, Ron M. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2011
Materias:
Application Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3177040/
https://www.ncbi.nlm.nih.gov/pubmed/21952901
http://dx.doi.org/10.1007/s13361-011-0203-z
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author Amstalden van Hove, Erika R.
Smith, Donald F.
Fornai, Lara
Glunde, Kristine
Heeren, Ron M. A.
author_facet Amstalden van Hove, Erika R.
Smith, Donald F.
Fornai, Lara
Glunde, Kristine
Heeren, Ron M. A.
author_sort Amstalden van Hove, Erika R.
collection PubMed
description Surface treatment of biological tissue sections improves detection of peptides and proteins for mass spectrometry imaging. However, liquid surface treatments can result in diffusion of surface analytes and fragile tissue sections can be easily damaged by typical washing solvents. Here, we present a new surface washing procedure for mass spectrometry imaging. This procedure uses solvent wetted fiber-free paper to enable local washing of tissue sections for mass spectrometry imaging and tissue profiling experiments. In addition, the method allows fragile tissues that cannot be treated by conventional washing techniques to be analyzed by mass spectrometry imaging.
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spelling pubmed-31770402011-09-30 An Alternative Paper Based Tissue Washing Method for Mass Spectrometry Imaging: Localized Washing and Fragile Tissue Analysis Amstalden van Hove, Erika R. Smith, Donald F. Fornai, Lara Glunde, Kristine Heeren, Ron M. A. J Am Soc Mass Spectrom Application Note Surface treatment of biological tissue sections improves detection of peptides and proteins for mass spectrometry imaging. However, liquid surface treatments can result in diffusion of surface analytes and fragile tissue sections can be easily damaged by typical washing solvents. Here, we present a new surface washing procedure for mass spectrometry imaging. This procedure uses solvent wetted fiber-free paper to enable local washing of tissue sections for mass spectrometry imaging and tissue profiling experiments. In addition, the method allows fragile tissues that cannot be treated by conventional washing techniques to be analyzed by mass spectrometry imaging. Springer-Verlag 2011-07-19 2011 /pmc/articles/PMC3177040/ /pubmed/21952901 http://dx.doi.org/10.1007/s13361-011-0203-z Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Application Note
Amstalden van Hove, Erika R.
Smith, Donald F.
Fornai, Lara
Glunde, Kristine
Heeren, Ron M. A.
An Alternative Paper Based Tissue Washing Method for Mass Spectrometry Imaging: Localized Washing and Fragile Tissue Analysis
title An Alternative Paper Based Tissue Washing Method for Mass Spectrometry Imaging: Localized Washing and Fragile Tissue Analysis
title_full An Alternative Paper Based Tissue Washing Method for Mass Spectrometry Imaging: Localized Washing and Fragile Tissue Analysis
title_fullStr An Alternative Paper Based Tissue Washing Method for Mass Spectrometry Imaging: Localized Washing and Fragile Tissue Analysis
title_full_unstemmed An Alternative Paper Based Tissue Washing Method for Mass Spectrometry Imaging: Localized Washing and Fragile Tissue Analysis
title_short An Alternative Paper Based Tissue Washing Method for Mass Spectrometry Imaging: Localized Washing and Fragile Tissue Analysis
title_sort alternative paper based tissue washing method for mass spectrometry imaging: localized washing and fragile tissue analysis
topic Application Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3177040/
https://www.ncbi.nlm.nih.gov/pubmed/21952901
http://dx.doi.org/10.1007/s13361-011-0203-z
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