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Simple and efficient synthesis of 5′ pre-adenylated DNA using thermostable RNA ligase

We report a simple method of enzymatic synthesis of pre-adenylated DNA linkers/adapters for next-generation sequencing using thermostable RNA ligase from Methanobacterium thermoautotrophicum (MthRnl). Using RNA ligase for the reaction instead of the existing chemical or T4 DNA ligase-based methods a...

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Detalles Bibliográficos
Autores principales:	Zhelkovsky, Alexander M., McReynolds, Larry A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2011
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3177227/ https://www.ncbi.nlm.nih.gov/pubmed/21724605 http://dx.doi.org/10.1093/nar/gkr544

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