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Pelvic Organ Distribution of Mesenchymal Stem Cells Injected Intravenously after Simulated Childbirth Injury in Female Rats

The local route of stem cell administration utilized presently in clinical trials for stress incontinence may not take full advantage of the capabilities of these cells. The goal of this study was to evaluate if intravenously injected mesenchymal stem cells (MSCs) home to pelvic organs after simulat...

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Autores principales: Cruz, Michelle, Dissaranan, Charuspong, Cotleur, Anne, Kiedrowski, Matthew, Penn, Marc, Damaser, Margot
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3177359/
https://www.ncbi.nlm.nih.gov/pubmed/21941558
http://dx.doi.org/10.1155/2012/612946
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author Cruz, Michelle
Dissaranan, Charuspong
Cotleur, Anne
Kiedrowski, Matthew
Penn, Marc
Damaser, Margot
author_facet Cruz, Michelle
Dissaranan, Charuspong
Cotleur, Anne
Kiedrowski, Matthew
Penn, Marc
Damaser, Margot
author_sort Cruz, Michelle
collection PubMed
description The local route of stem cell administration utilized presently in clinical trials for stress incontinence may not take full advantage of the capabilities of these cells. The goal of this study was to evaluate if intravenously injected mesenchymal stem cells (MSCs) home to pelvic organs after simulated childbirth injury in a rat model. Female rats underwent either vaginal distension (VD) or sham VD. All rats received 2 million GFP-labeled MSCs intravenously 1 hour after injury. Four or 10 days later pelvic organs and muscles were imaged for visualization of GFP-positive cells. Significantly more MSCs home to the urethra, vagina, rectum, and levator ani muscle 4 days after VD than after sham VD. MSCs were present 10 days after injection but GFP intensity had decreased. This study provides basic science evidence that intravenous administration of MSCs could provide an effective route for cell-based therapy to facilitate repair after injury and treat stress incontinence.
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spelling pubmed-31773592011-09-22 Pelvic Organ Distribution of Mesenchymal Stem Cells Injected Intravenously after Simulated Childbirth Injury in Female Rats Cruz, Michelle Dissaranan, Charuspong Cotleur, Anne Kiedrowski, Matthew Penn, Marc Damaser, Margot Obstet Gynecol Int Research Article The local route of stem cell administration utilized presently in clinical trials for stress incontinence may not take full advantage of the capabilities of these cells. The goal of this study was to evaluate if intravenously injected mesenchymal stem cells (MSCs) home to pelvic organs after simulated childbirth injury in a rat model. Female rats underwent either vaginal distension (VD) or sham VD. All rats received 2 million GFP-labeled MSCs intravenously 1 hour after injury. Four or 10 days later pelvic organs and muscles were imaged for visualization of GFP-positive cells. Significantly more MSCs home to the urethra, vagina, rectum, and levator ani muscle 4 days after VD than after sham VD. MSCs were present 10 days after injection but GFP intensity had decreased. This study provides basic science evidence that intravenous administration of MSCs could provide an effective route for cell-based therapy to facilitate repair after injury and treat stress incontinence. Hindawi Publishing Corporation 2012 2011-09-21 /pmc/articles/PMC3177359/ /pubmed/21941558 http://dx.doi.org/10.1155/2012/612946 Text en Copyright © 2012 Michelle Cruz et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cruz, Michelle
Dissaranan, Charuspong
Cotleur, Anne
Kiedrowski, Matthew
Penn, Marc
Damaser, Margot
Pelvic Organ Distribution of Mesenchymal Stem Cells Injected Intravenously after Simulated Childbirth Injury in Female Rats
title Pelvic Organ Distribution of Mesenchymal Stem Cells Injected Intravenously after Simulated Childbirth Injury in Female Rats
title_full Pelvic Organ Distribution of Mesenchymal Stem Cells Injected Intravenously after Simulated Childbirth Injury in Female Rats
title_fullStr Pelvic Organ Distribution of Mesenchymal Stem Cells Injected Intravenously after Simulated Childbirth Injury in Female Rats
title_full_unstemmed Pelvic Organ Distribution of Mesenchymal Stem Cells Injected Intravenously after Simulated Childbirth Injury in Female Rats
title_short Pelvic Organ Distribution of Mesenchymal Stem Cells Injected Intravenously after Simulated Childbirth Injury in Female Rats
title_sort pelvic organ distribution of mesenchymal stem cells injected intravenously after simulated childbirth injury in female rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3177359/
https://www.ncbi.nlm.nih.gov/pubmed/21941558
http://dx.doi.org/10.1155/2012/612946
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