High Refractive Index Silicone Gels for Simultaneous Total Internal Reflection Fluorescence and Traction Force Microscopy of Adherent Cells

Substrate rigidity profoundly impacts cellular behaviors such as migration, gene expression, and cell fate. Total Internal Reflection Fluorescence (TIRF) microscopy enables selective visualization of the dynamics of substrate adhesions, vesicle trafficking, and biochemical signaling at the cell-subs...

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Autores principales: Gutierrez, Edgar, Tkachenko, Eugene, Besser, Achim, Sundd, Prithu, Ley, Klaus, Danuser, Gaudenz, Ginsberg, Mark H., Groisman, Alex
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3178539/
https://www.ncbi.nlm.nih.gov/pubmed/21961031
http://dx.doi.org/10.1371/journal.pone.0023807
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author Gutierrez, Edgar
Tkachenko, Eugene
Besser, Achim
Sundd, Prithu
Ley, Klaus
Danuser, Gaudenz
Ginsberg, Mark H.
Groisman, Alex
author_facet Gutierrez, Edgar
Tkachenko, Eugene
Besser, Achim
Sundd, Prithu
Ley, Klaus
Danuser, Gaudenz
Ginsberg, Mark H.
Groisman, Alex
author_sort Gutierrez, Edgar
collection PubMed
description Substrate rigidity profoundly impacts cellular behaviors such as migration, gene expression, and cell fate. Total Internal Reflection Fluorescence (TIRF) microscopy enables selective visualization of the dynamics of substrate adhesions, vesicle trafficking, and biochemical signaling at the cell-substrate interface. Here we apply high-refractive-index silicone gels to perform TIRF microscopy on substrates with a wide range of physiological elastic moduli and simultaneously measure traction forces exerted by cells on the substrate.
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spelling pubmed-31785392011-09-29 High Refractive Index Silicone Gels for Simultaneous Total Internal Reflection Fluorescence and Traction Force Microscopy of Adherent Cells Gutierrez, Edgar Tkachenko, Eugene Besser, Achim Sundd, Prithu Ley, Klaus Danuser, Gaudenz Ginsberg, Mark H. Groisman, Alex PLoS One Research Article Substrate rigidity profoundly impacts cellular behaviors such as migration, gene expression, and cell fate. Total Internal Reflection Fluorescence (TIRF) microscopy enables selective visualization of the dynamics of substrate adhesions, vesicle trafficking, and biochemical signaling at the cell-substrate interface. Here we apply high-refractive-index silicone gels to perform TIRF microscopy on substrates with a wide range of physiological elastic moduli and simultaneously measure traction forces exerted by cells on the substrate. Public Library of Science 2011-09-22 /pmc/articles/PMC3178539/ /pubmed/21961031 http://dx.doi.org/10.1371/journal.pone.0023807 Text en Gutierrez et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Gutierrez, Edgar
Tkachenko, Eugene
Besser, Achim
Sundd, Prithu
Ley, Klaus
Danuser, Gaudenz
Ginsberg, Mark H.
Groisman, Alex
High Refractive Index Silicone Gels for Simultaneous Total Internal Reflection Fluorescence and Traction Force Microscopy of Adherent Cells
title High Refractive Index Silicone Gels for Simultaneous Total Internal Reflection Fluorescence and Traction Force Microscopy of Adherent Cells
title_full High Refractive Index Silicone Gels for Simultaneous Total Internal Reflection Fluorescence and Traction Force Microscopy of Adherent Cells
title_fullStr High Refractive Index Silicone Gels for Simultaneous Total Internal Reflection Fluorescence and Traction Force Microscopy of Adherent Cells
title_full_unstemmed High Refractive Index Silicone Gels for Simultaneous Total Internal Reflection Fluorescence and Traction Force Microscopy of Adherent Cells
title_short High Refractive Index Silicone Gels for Simultaneous Total Internal Reflection Fluorescence and Traction Force Microscopy of Adherent Cells
title_sort high refractive index silicone gels for simultaneous total internal reflection fluorescence and traction force microscopy of adherent cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3178539/
https://www.ncbi.nlm.nih.gov/pubmed/21961031
http://dx.doi.org/10.1371/journal.pone.0023807
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