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Towards Protein Crystallization as a Process Step in Downstream Processing of Therapeutic Antibodies: Screening and Optimization at Microbatch Scale

Crystallization conditions of an intact monoclonal IgG4 (immunoglobulin G, subclass 4) antibody were established in vapor diffusion mode by sparse matrix screening and subsequent optimization. The procedure was transferred to microbatch conditions and a phase diagram was built showing surprisingly l...

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Detalles Bibliográficos
Autores principales:	Zang, Yuguo, Kammerer, Bernd, Eisenkolb, Maike, Lohr, Katrin, Kiefer, Hans
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2011
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3178630/ https://www.ncbi.nlm.nih.gov/pubmed/21966480 http://dx.doi.org/10.1371/journal.pone.0025282

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