Cargando…

Determination of Actarit from Human Plasma for Bioequivalence Studies

An analytical method based on high-performance liquid chromatography with ultraviolet detection (245 nm) was developed for the determination of actarit in human plasma. Coumarin was used as an internal standard. Chromatographic separation was achieved with a C8 column using a mobile phase of methano...

Descripción completa

Detalles Bibliográficos
Autores principales: Loya, P., Saraf, M. N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3178973/
https://www.ncbi.nlm.nih.gov/pubmed/21969744
http://dx.doi.org/10.4103/0250-474X.84581
_version_ 1782212469919318016
author Loya, P.
Saraf, M. N.
author_facet Loya, P.
Saraf, M. N.
author_sort Loya, P.
collection PubMed
description An analytical method based on high-performance liquid chromatography with ultraviolet detection (245 nm) was developed for the determination of actarit in human plasma. Coumarin was used as an internal standard. Chromatographic separation was achieved with a C8 column using a mobile phase of methanol: 1% acetic acid (50-50, v/v) with a flow rate of 1.0 ml/min. The calibration curve was linear over the range of 0.1–4.0 μg/ml (r(2) > 0.99) and the lower limit of quantification was 0.1 μg/ml. The method was validated for sensitivity, accuracy, precision, recovery and stability. The method was used to determine the concentration-time profiles of actarit in the plasma following oral administration of 100 mg actarit tablets.
format Online
Article
Text
id pubmed-3178973
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher Medknow Publications
record_format MEDLINE/PubMed
spelling pubmed-31789732011-10-03 Determination of Actarit from Human Plasma for Bioequivalence Studies Loya, P. Saraf, M. N. Indian J Pharm Sci Research Paper An analytical method based on high-performance liquid chromatography with ultraviolet detection (245 nm) was developed for the determination of actarit in human plasma. Coumarin was used as an internal standard. Chromatographic separation was achieved with a C8 column using a mobile phase of methanol: 1% acetic acid (50-50, v/v) with a flow rate of 1.0 ml/min. The calibration curve was linear over the range of 0.1–4.0 μg/ml (r(2) > 0.99) and the lower limit of quantification was 0.1 μg/ml. The method was validated for sensitivity, accuracy, precision, recovery and stability. The method was used to determine the concentration-time profiles of actarit in the plasma following oral administration of 100 mg actarit tablets. Medknow Publications 2010 /pmc/articles/PMC3178973/ /pubmed/21969744 http://dx.doi.org/10.4103/0250-474X.84581 Text en Copyright: © Indian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Loya, P.
Saraf, M. N.
Determination of Actarit from Human Plasma for Bioequivalence Studies
title Determination of Actarit from Human Plasma for Bioequivalence Studies
title_full Determination of Actarit from Human Plasma for Bioequivalence Studies
title_fullStr Determination of Actarit from Human Plasma for Bioequivalence Studies
title_full_unstemmed Determination of Actarit from Human Plasma for Bioequivalence Studies
title_short Determination of Actarit from Human Plasma for Bioequivalence Studies
title_sort determination of actarit from human plasma for bioequivalence studies
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3178973/
https://www.ncbi.nlm.nih.gov/pubmed/21969744
http://dx.doi.org/10.4103/0250-474X.84581
work_keys_str_mv AT loyap determinationofactaritfromhumanplasmaforbioequivalencestudies
AT sarafmn determinationofactaritfromhumanplasmaforbioequivalencestudies