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Analysis of 16S rRNA Amplicon Sequencing Options on the Roche/454 Next-Generation Titanium Sequencing Platform

BACKGROUND: 16S rRNA gene pyrosequencing approach has revolutionized studies in microbial ecology. While primer selection and short read length can affect the resulting microbial community profile, little is known about the influence of pyrosequencing methods on the sequencing throughput and the out...

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Detalles Bibliográficos
Autores principales:	Tamaki, Hideyuki, Wright, Chris L., Li, Xiangzhen, Lin, Qiaoyan, Hwang, Chiachi, Wang, Shiping, Thimmapuram, Jyothi, Kamagata, Yoichi, Liu, Wen-Tso
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2011
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3179495/ https://www.ncbi.nlm.nih.gov/pubmed/21966473 http://dx.doi.org/10.1371/journal.pone.0025263

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