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Vaginal Microbiota of Healthy Pregnant Mexican Women is Constituted by Four Lactobacillus Species and Several Vaginosis-Associated Bacteria

Objective. To identify the microbiota communities in the vaginal tracts of healthy Mexican women across the pregnancy. Methods. Vaginal swabs were obtained during the prenatal visit of women from all trimesters (n = 64) of healthy pregnant women of Mexico City. DNA was isolated from each sample, and...

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Autores principales: Hernández-Rodríguez, César, Romero-González, Roberto, Albani-Campanario, Mario, Figueroa-Damián, Ricardo, Meraz-Cruz, Noemí, Hernández-Guerrero, César
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3179877/
https://www.ncbi.nlm.nih.gov/pubmed/21960733
http://dx.doi.org/10.1155/2011/851485
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author Hernández-Rodríguez, César
Romero-González, Roberto
Albani-Campanario, Mario
Figueroa-Damián, Ricardo
Meraz-Cruz, Noemí
Hernández-Guerrero, César
author_facet Hernández-Rodríguez, César
Romero-González, Roberto
Albani-Campanario, Mario
Figueroa-Damián, Ricardo
Meraz-Cruz, Noemí
Hernández-Guerrero, César
author_sort Hernández-Rodríguez, César
collection PubMed
description Objective. To identify the microbiota communities in the vaginal tracts of healthy Mexican women across the pregnancy. Methods. Vaginal swabs were obtained during the prenatal visit of women from all trimesters (n = 64) of healthy pregnant women of Mexico City. DNA was isolated from each sample, and PCR-DGGE and sequencing of 16S rRNA gene fragments were used to identify the bacterial communities. Results. 21 different microorganisms were identified in the vaginal samples. Lactobacillus genus was present in 98% of women studied. Four lactobacilli species were identified in vaginal samples. L. acidophilus was the predominant (78%) followed by L. iners (54%), L. gasseri (20%), and L. delbrueckii (6%). 17 different microorganisms related to bacterial vaginosis conditions were identified. Ureaplasma urealyticum was the predominant (21%) followed by BVAB1 (17%) and Gemella bergeriae (7.8%). Conclusions. Lactobacillus genus predominates in the vaginal samples of Mexican pregnant women associated with different microorganisms related to bacterial vaginosis conditions.
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spelling pubmed-31798772011-09-29 Vaginal Microbiota of Healthy Pregnant Mexican Women is Constituted by Four Lactobacillus Species and Several Vaginosis-Associated Bacteria Hernández-Rodríguez, César Romero-González, Roberto Albani-Campanario, Mario Figueroa-Damián, Ricardo Meraz-Cruz, Noemí Hernández-Guerrero, César Infect Dis Obstet Gynecol Research Article Objective. To identify the microbiota communities in the vaginal tracts of healthy Mexican women across the pregnancy. Methods. Vaginal swabs were obtained during the prenatal visit of women from all trimesters (n = 64) of healthy pregnant women of Mexico City. DNA was isolated from each sample, and PCR-DGGE and sequencing of 16S rRNA gene fragments were used to identify the bacterial communities. Results. 21 different microorganisms were identified in the vaginal samples. Lactobacillus genus was present in 98% of women studied. Four lactobacilli species were identified in vaginal samples. L. acidophilus was the predominant (78%) followed by L. iners (54%), L. gasseri (20%), and L. delbrueckii (6%). 17 different microorganisms related to bacterial vaginosis conditions were identified. Ureaplasma urealyticum was the predominant (21%) followed by BVAB1 (17%) and Gemella bergeriae (7.8%). Conclusions. Lactobacillus genus predominates in the vaginal samples of Mexican pregnant women associated with different microorganisms related to bacterial vaginosis conditions. Hindawi Publishing Corporation 2011 2011-09-22 /pmc/articles/PMC3179877/ /pubmed/21960733 http://dx.doi.org/10.1155/2011/851485 Text en Copyright © 2011 César Hernández-Rodríguez et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Hernández-Rodríguez, César
Romero-González, Roberto
Albani-Campanario, Mario
Figueroa-Damián, Ricardo
Meraz-Cruz, Noemí
Hernández-Guerrero, César
Vaginal Microbiota of Healthy Pregnant Mexican Women is Constituted by Four Lactobacillus Species and Several Vaginosis-Associated Bacteria
title Vaginal Microbiota of Healthy Pregnant Mexican Women is Constituted by Four Lactobacillus Species and Several Vaginosis-Associated Bacteria
title_full Vaginal Microbiota of Healthy Pregnant Mexican Women is Constituted by Four Lactobacillus Species and Several Vaginosis-Associated Bacteria
title_fullStr Vaginal Microbiota of Healthy Pregnant Mexican Women is Constituted by Four Lactobacillus Species and Several Vaginosis-Associated Bacteria
title_full_unstemmed Vaginal Microbiota of Healthy Pregnant Mexican Women is Constituted by Four Lactobacillus Species and Several Vaginosis-Associated Bacteria
title_short Vaginal Microbiota of Healthy Pregnant Mexican Women is Constituted by Four Lactobacillus Species and Several Vaginosis-Associated Bacteria
title_sort vaginal microbiota of healthy pregnant mexican women is constituted by four lactobacillus species and several vaginosis-associated bacteria
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3179877/
https://www.ncbi.nlm.nih.gov/pubmed/21960733
http://dx.doi.org/10.1155/2011/851485
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