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Enzymatic synthesis of long double-stranded DNA labeled with haloderivatives of nucleobases in a precisely pre-determined sequence

BACKGROUND: Restriction endonucleases are widely applied in recombinant DNA technology. Among them, enzymes of class IIS, which cleave DNA beyond recognition sites, are especially useful. We use BsaI enzyme for the pinpoint introduction of halogen nucleobases into DNA. This has been done for the pur...

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Detalles Bibliográficos
Autores principales:	Sobolewski, Ireneusz, Polska, Katarzyna, Żylicz-Stachula, Agnieszka, Jeżewska-Frąckowiak, Joanna, Rak, Janusz, Skowron, Piotr
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2011
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3179937/ https://www.ncbi.nlm.nih.gov/pubmed/21864341 http://dx.doi.org/10.1186/1471-2091-12-47

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