Use of BODIPY (493/503) to Visualize Intramuscular Lipid Droplets in Skeletal Muscle

Triglyceride storage is altered across various chronic health conditions necessitating various techniques to visualize and quantify lipid droplets (LDs). Here, we describe the utilization of the BODIPY (493/503) dye in skeletal muscle as a means to analyze LDs. We found that the dye was a convenient...

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Detalles Bibliográficos
Autores principales: Spangenburg, Espen E., Pratt, Stephen J. P., Wohlers, Lindsay M., Lovering, Richard M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Methodology Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3180081/
https://www.ncbi.nlm.nih.gov/pubmed/21960738
http://dx.doi.org/10.1155/2011/598358
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author Spangenburg, Espen E.
Pratt, Stephen J. P.
Wohlers, Lindsay M.
Lovering, Richard M.
author_facet Spangenburg, Espen E.
Pratt, Stephen J. P.
Wohlers, Lindsay M.
Lovering, Richard M.
author_sort Spangenburg, Espen E.
collection PubMed
description Triglyceride storage is altered across various chronic health conditions necessitating various techniques to visualize and quantify lipid droplets (LDs). Here, we describe the utilization of the BODIPY (493/503) dye in skeletal muscle as a means to analyze LDs. We found that the dye was a convenient and simple approach to visualize LDs in both sectioned skeletal muscle and cultured adult single fibers. Furthermore, the dye was effective in both fixed and nonfixed cells, and the staining seemed unaffected by permeabilization. We believe that the use of the BODIPY (493/503) dye is an acceptable alternative and, under certain conditions, a simpler method for visualizing LDs stored within skeletal muscle.
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spelling pubmed-31800812011-09-29 Use of BODIPY (493/503) to Visualize Intramuscular Lipid Droplets in Skeletal Muscle Spangenburg, Espen E. Pratt, Stephen J. P. Wohlers, Lindsay M. Lovering, Richard M. J Biomed Biotechnol Methodology Report Triglyceride storage is altered across various chronic health conditions necessitating various techniques to visualize and quantify lipid droplets (LDs). Here, we describe the utilization of the BODIPY (493/503) dye in skeletal muscle as a means to analyze LDs. We found that the dye was a convenient and simple approach to visualize LDs in both sectioned skeletal muscle and cultured adult single fibers. Furthermore, the dye was effective in both fixed and nonfixed cells, and the staining seemed unaffected by permeabilization. We believe that the use of the BODIPY (493/503) dye is an acceptable alternative and, under certain conditions, a simpler method for visualizing LDs stored within skeletal muscle. Hindawi Publishing Corporation 2011 2011-09-25 /pmc/articles/PMC3180081/ /pubmed/21960738 http://dx.doi.org/10.1155/2011/598358 Text en Copyright © 2011 Espen E. Spangenburg et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology Report
Spangenburg, Espen E.
Pratt, Stephen J. P.
Wohlers, Lindsay M.
Lovering, Richard M.
Use of BODIPY (493/503) to Visualize Intramuscular Lipid Droplets in Skeletal Muscle
title Use of BODIPY (493/503) to Visualize Intramuscular Lipid Droplets in Skeletal Muscle
title_full Use of BODIPY (493/503) to Visualize Intramuscular Lipid Droplets in Skeletal Muscle
title_fullStr Use of BODIPY (493/503) to Visualize Intramuscular Lipid Droplets in Skeletal Muscle
title_full_unstemmed Use of BODIPY (493/503) to Visualize Intramuscular Lipid Droplets in Skeletal Muscle
title_short Use of BODIPY (493/503) to Visualize Intramuscular Lipid Droplets in Skeletal Muscle
title_sort use of bodipy (493/503) to visualize intramuscular lipid droplets in skeletal muscle
topic Methodology Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3180081/
https://www.ncbi.nlm.nih.gov/pubmed/21960738
http://dx.doi.org/10.1155/2011/598358
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