Comparative Hydrogen–Deuterium Exchange for a Mesophilic vs Thermophilic Dihydrofolate Reductase at 25 °C: Identification of a Single Active Site Region with Enhanced Flexibility in the Mesophilic Protein

[Image: see text] The technique of hydrogen–deuterium exchange coupled to mass spectrometry (HDX-MS) has been applied to a mesophilic (E. coli) dihydrofolate reductase under conditions that allow direct comparison to a thermophilic (B. stearothermophilus) ortholog, Ec-DHFR and Bs-DHFR, respectively....

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Detalles Bibliográficos
Autores principales: Oyeyemi, Olayinka A., Sours, Kevin M., Lee, Thomas, Kohen, Amnon, Resing, Katheryn A., Ahn, Natalie G., Klinman, Judith P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2011
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3180199/
https://www.ncbi.nlm.nih.gov/pubmed/21859100
http://dx.doi.org/10.1021/bi200640s

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