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Comparative Genomics Study of Multi-Drug-Resistance Mechanisms in the Antibiotic-Resistant Streptococcus suis R61 Strain

BACKGROUND: Streptococcus suis infections are a serious problem for both humans and pigs worldwide. The emergence and increasing prevalence of antibiotic-resistant S. suis strains pose significant clinical and societal challenges. RESULTS: In our study, we sequenced one multi-drug-resistant S. suis...

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Autores principales: Hu, Pan, Yang, Ming, Zhang, Anding, Wu, Jiayan, Chen, Bo, Hua, Yafeng, Yu, Jun, Chen, Huanchun, Xiao, Jingfa, Jin, Meilin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3180280/
https://www.ncbi.nlm.nih.gov/pubmed/21966396
http://dx.doi.org/10.1371/journal.pone.0024988
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author Hu, Pan
Yang, Ming
Zhang, Anding
Wu, Jiayan
Chen, Bo
Hua, Yafeng
Yu, Jun
Chen, Huanchun
Xiao, Jingfa
Jin, Meilin
author_facet Hu, Pan
Yang, Ming
Zhang, Anding
Wu, Jiayan
Chen, Bo
Hua, Yafeng
Yu, Jun
Chen, Huanchun
Xiao, Jingfa
Jin, Meilin
author_sort Hu, Pan
collection PubMed
description BACKGROUND: Streptococcus suis infections are a serious problem for both humans and pigs worldwide. The emergence and increasing prevalence of antibiotic-resistant S. suis strains pose significant clinical and societal challenges. RESULTS: In our study, we sequenced one multi-drug-resistant S. suis strain, R61, and one S. suis strain, A7, which is fully sensitive to all tested antibiotics. Comparative genomic analysis revealed that the R61 strain is phylogenetically distinct from other S. suis strains, and the genome of R61 exhibits extreme levels of evolutionary plasticity with high levels of gene gain and loss. Our results indicate that the multi-drug-resistant strain R61 has evolved three main categories of resistance. CONCLUSIONS: Comparative genomic analysis of S. suis strains with diverse drug-resistant phenotypes provided evidence that horizontal gene transfer is an important evolutionary force in shaping the genome of multi-drug-resistant strain R61. In this study, we discovered novel and previously unexamined mutations that are strong candidates for conferring drug resistance. We believe that these mutations will provide crucial clues for designing new drugs against this pathogen. In addition, our work provides a clear demonstration that the use of drugs has driven the emergence of the multi-drug-resistant strain R61.
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spelling pubmed-31802802011-09-30 Comparative Genomics Study of Multi-Drug-Resistance Mechanisms in the Antibiotic-Resistant Streptococcus suis R61 Strain Hu, Pan Yang, Ming Zhang, Anding Wu, Jiayan Chen, Bo Hua, Yafeng Yu, Jun Chen, Huanchun Xiao, Jingfa Jin, Meilin PLoS One Research Article BACKGROUND: Streptococcus suis infections are a serious problem for both humans and pigs worldwide. The emergence and increasing prevalence of antibiotic-resistant S. suis strains pose significant clinical and societal challenges. RESULTS: In our study, we sequenced one multi-drug-resistant S. suis strain, R61, and one S. suis strain, A7, which is fully sensitive to all tested antibiotics. Comparative genomic analysis revealed that the R61 strain is phylogenetically distinct from other S. suis strains, and the genome of R61 exhibits extreme levels of evolutionary plasticity with high levels of gene gain and loss. Our results indicate that the multi-drug-resistant strain R61 has evolved three main categories of resistance. CONCLUSIONS: Comparative genomic analysis of S. suis strains with diverse drug-resistant phenotypes provided evidence that horizontal gene transfer is an important evolutionary force in shaping the genome of multi-drug-resistant strain R61. In this study, we discovered novel and previously unexamined mutations that are strong candidates for conferring drug resistance. We believe that these mutations will provide crucial clues for designing new drugs against this pathogen. In addition, our work provides a clear demonstration that the use of drugs has driven the emergence of the multi-drug-resistant strain R61. Public Library of Science 2011-09-26 /pmc/articles/PMC3180280/ /pubmed/21966396 http://dx.doi.org/10.1371/journal.pone.0024988 Text en Hu et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hu, Pan
Yang, Ming
Zhang, Anding
Wu, Jiayan
Chen, Bo
Hua, Yafeng
Yu, Jun
Chen, Huanchun
Xiao, Jingfa
Jin, Meilin
Comparative Genomics Study of Multi-Drug-Resistance Mechanisms in the Antibiotic-Resistant Streptococcus suis R61 Strain
title Comparative Genomics Study of Multi-Drug-Resistance Mechanisms in the Antibiotic-Resistant Streptococcus suis R61 Strain
title_full Comparative Genomics Study of Multi-Drug-Resistance Mechanisms in the Antibiotic-Resistant Streptococcus suis R61 Strain
title_fullStr Comparative Genomics Study of Multi-Drug-Resistance Mechanisms in the Antibiotic-Resistant Streptococcus suis R61 Strain
title_full_unstemmed Comparative Genomics Study of Multi-Drug-Resistance Mechanisms in the Antibiotic-Resistant Streptococcus suis R61 Strain
title_short Comparative Genomics Study of Multi-Drug-Resistance Mechanisms in the Antibiotic-Resistant Streptococcus suis R61 Strain
title_sort comparative genomics study of multi-drug-resistance mechanisms in the antibiotic-resistant streptococcus suis r61 strain
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3180280/
https://www.ncbi.nlm.nih.gov/pubmed/21966396
http://dx.doi.org/10.1371/journal.pone.0024988
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