Origin and removal of mixed-phase artifacts in gradient sensitivity enhanced heteronuclear single quantum correlation spectra

Here we describe phasing anomalies observed in gradient sensitivity enhanced (15)N-(1)H HSQC spectra, and analyze their origin. It is shown that, as a result of (15)N off-resonance effects, dispersive contributions to the (1)H signal become detectable, and lead to (15)N-offset dependent phase errors...

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Detalles Bibliográficos
Autores principales: Mulder, Frans A. A., Otten, Renee, Scheek, Ruud M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3180630/
https://www.ncbi.nlm.nih.gov/pubmed/21947928
http://dx.doi.org/10.1007/s10858-011-9554-9
Descripción
Sumario:Here we describe phasing anomalies observed in gradient sensitivity enhanced (15)N-(1)H HSQC spectra, and analyze their origin. It is shown that, as a result of (15)N off-resonance effects, dispersive contributions to the (1)H signal become detectable, and lead to (15)N-offset dependent phase errors. Strategies that effectively suppress these artifacts are presented.

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