Origin and removal of mixed-phase artifacts in gradient sensitivity enhanced heteronuclear single quantum correlation spectra

Here we describe phasing anomalies observed in gradient sensitivity enhanced (15)N-(1)H HSQC spectra, and analyze their origin. It is shown that, as a result of (15)N off-resonance effects, dispersive contributions to the (1)H signal become detectable, and lead to (15)N-offset dependent phase errors...

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Detalles Bibliográficos
Autores principales: Mulder, Frans A. A., Otten, Renee, Scheek, Ruud M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3180630/
https://www.ncbi.nlm.nih.gov/pubmed/21947928
http://dx.doi.org/10.1007/s10858-011-9554-9
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author Mulder, Frans A. A.
Otten, Renee
Scheek, Ruud M.
author_facet Mulder, Frans A. A.
Otten, Renee
Scheek, Ruud M.
author_sort Mulder, Frans A. A.
collection PubMed
description Here we describe phasing anomalies observed in gradient sensitivity enhanced (15)N-(1)H HSQC spectra, and analyze their origin. It is shown that, as a result of (15)N off-resonance effects, dispersive contributions to the (1)H signal become detectable, and lead to (15)N-offset dependent phase errors. Strategies that effectively suppress these artifacts are presented.
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spelling pubmed-31806302011-10-04 Origin and removal of mixed-phase artifacts in gradient sensitivity enhanced heteronuclear single quantum correlation spectra Mulder, Frans A. A. Otten, Renee Scheek, Ruud M. J Biomol NMR Article Here we describe phasing anomalies observed in gradient sensitivity enhanced (15)N-(1)H HSQC spectra, and analyze their origin. It is shown that, as a result of (15)N off-resonance effects, dispersive contributions to the (1)H signal become detectable, and lead to (15)N-offset dependent phase errors. Strategies that effectively suppress these artifacts are presented. Springer Netherlands 2011-09-27 2011 /pmc/articles/PMC3180630/ /pubmed/21947928 http://dx.doi.org/10.1007/s10858-011-9554-9 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Article
Mulder, Frans A. A.
Otten, Renee
Scheek, Ruud M.
Origin and removal of mixed-phase artifacts in gradient sensitivity enhanced heteronuclear single quantum correlation spectra
title Origin and removal of mixed-phase artifacts in gradient sensitivity enhanced heteronuclear single quantum correlation spectra
title_full Origin and removal of mixed-phase artifacts in gradient sensitivity enhanced heteronuclear single quantum correlation spectra
title_fullStr Origin and removal of mixed-phase artifacts in gradient sensitivity enhanced heteronuclear single quantum correlation spectra
title_full_unstemmed Origin and removal of mixed-phase artifacts in gradient sensitivity enhanced heteronuclear single quantum correlation spectra
title_short Origin and removal of mixed-phase artifacts in gradient sensitivity enhanced heteronuclear single quantum correlation spectra
title_sort origin and removal of mixed-phase artifacts in gradient sensitivity enhanced heteronuclear single quantum correlation spectra
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3180630/
https://www.ncbi.nlm.nih.gov/pubmed/21947928
http://dx.doi.org/10.1007/s10858-011-9554-9
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