A Proteome Reference Map of the Causative Agent of Melioidosis Burkholderia pseudomallei

Burkholderia pseudomallei is the etiologic agent of melioidosis. Using 2DE and MALDI-TOF MS, we report here a proteome reference map constructed from early stationary phase, a bacterial adaptation process. We identified 282 protein spots representing 220 ORFs; many of them have been implicated in ba...

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Detalles Bibliográficos
Autores principales: Wongtrakoongate, Patompon, Roytrakul, Sittiruk, Yasothornsrikul, Sukkid, Tungpradabkul, Sumalee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3180641/
https://www.ncbi.nlm.nih.gov/pubmed/21960737
http://dx.doi.org/10.1155/2011/530926
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author Wongtrakoongate, Patompon
Roytrakul, Sittiruk
Yasothornsrikul, Sukkid
Tungpradabkul, Sumalee
author_facet Wongtrakoongate, Patompon
Roytrakul, Sittiruk
Yasothornsrikul, Sukkid
Tungpradabkul, Sumalee
author_sort Wongtrakoongate, Patompon
collection PubMed
description Burkholderia pseudomallei is the etiologic agent of melioidosis. Using 2DE and MALDI-TOF MS, we report here a proteome reference map constructed from early stationary phase, a bacterial adaptation process. We identified 282 protein spots representing 220 ORFs; many of them have been implicated in bacterial pathogenesis. Up to 20% of identified ORFs belong to post-translational modification and stress responses. The proteome reference map will support future analysis of the bacterial gene and environmental regulation and facilitate comparative proteomics with its sibling species.
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spelling pubmed-31806412011-09-29 A Proteome Reference Map of the Causative Agent of Melioidosis Burkholderia pseudomallei Wongtrakoongate, Patompon Roytrakul, Sittiruk Yasothornsrikul, Sukkid Tungpradabkul, Sumalee J Biomed Biotechnol Research Article Burkholderia pseudomallei is the etiologic agent of melioidosis. Using 2DE and MALDI-TOF MS, we report here a proteome reference map constructed from early stationary phase, a bacterial adaptation process. We identified 282 protein spots representing 220 ORFs; many of them have been implicated in bacterial pathogenesis. Up to 20% of identified ORFs belong to post-translational modification and stress responses. The proteome reference map will support future analysis of the bacterial gene and environmental regulation and facilitate comparative proteomics with its sibling species. Hindawi Publishing Corporation 2011 2011-09-22 /pmc/articles/PMC3180641/ /pubmed/21960737 http://dx.doi.org/10.1155/2011/530926 Text en Copyright © 2011 Patompon Wongtrakoongate et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wongtrakoongate, Patompon
Roytrakul, Sittiruk
Yasothornsrikul, Sukkid
Tungpradabkul, Sumalee
A Proteome Reference Map of the Causative Agent of Melioidosis Burkholderia pseudomallei
title A Proteome Reference Map of the Causative Agent of Melioidosis Burkholderia pseudomallei
title_full A Proteome Reference Map of the Causative Agent of Melioidosis Burkholderia pseudomallei
title_fullStr A Proteome Reference Map of the Causative Agent of Melioidosis Burkholderia pseudomallei
title_full_unstemmed A Proteome Reference Map of the Causative Agent of Melioidosis Burkholderia pseudomallei
title_short A Proteome Reference Map of the Causative Agent of Melioidosis Burkholderia pseudomallei
title_sort proteome reference map of the causative agent of melioidosis burkholderia pseudomallei
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3180641/
https://www.ncbi.nlm.nih.gov/pubmed/21960737
http://dx.doi.org/10.1155/2011/530926
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