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Monilinia Species Causing Brown Rot of Peach in China

In this study, 145 peaches and nectarines displaying typical brown rot symptoms were collected from multiple provinces in China. A subsample of 26 single-spore isolates were characterized phylogenetically and morphologically to ascertain species. Phylogenetic analysis of internal transcribed spacer...

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Autores principales: Hu, Meng-Jun, Cox, Kerik D., Schnabel, Guido, Luo, Chao-Xi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3181254/
https://www.ncbi.nlm.nih.gov/pubmed/21980371
http://dx.doi.org/10.1371/journal.pone.0024990
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author Hu, Meng-Jun
Cox, Kerik D.
Schnabel, Guido
Luo, Chao-Xi
author_facet Hu, Meng-Jun
Cox, Kerik D.
Schnabel, Guido
Luo, Chao-Xi
author_sort Hu, Meng-Jun
collection PubMed
description In this study, 145 peaches and nectarines displaying typical brown rot symptoms were collected from multiple provinces in China. A subsample of 26 single-spore isolates were characterized phylogenetically and morphologically to ascertain species. Phylogenetic analysis of internal transcribed spacer (ITS) regions 1 and 2, glyceraldehyde-3-phosphate dehydrogenase (G3PDH), β-tubulin (TUB2) revealed the presence of three distinct Monilinia species. These species included Monilinia fructicola, Monilia mumecola, and a previously undescribed species designated Monilia yunnanensis sp. nov. While M. fructicola is a well-documented pathogen of Prunus persica in China, M. mumecola had primarily only been isolated from mume fruit in Japan. Koch's postulates for M. mumecola and M. yunnanensis were fulfilled confirming pathogenicity of the two species on peach. Phylogenetic analysis of ITS, G3PDH, and TUB2 sequences indicated that M. yunnanensis is most closely related to M. fructigena, a species widely prevalent in Europe. Interestingly, there were considerable differences in the exon/intron structure of the cytochrome b (Cyt b) gene between the two species. Morphological characteristics, including spore size, colony morphology, lesion growth rate, and sporulation, support the phylogenetic evidence suggesting the designation of M. yunnanensis as a new species. A new multiplex PCR method was developed to facilitate the detection of M. yunnanensis and differentiation of Monilinia spp. causing brown rot of peach in China.
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spelling pubmed-31812542011-10-06 Monilinia Species Causing Brown Rot of Peach in China Hu, Meng-Jun Cox, Kerik D. Schnabel, Guido Luo, Chao-Xi PLoS One Research Article In this study, 145 peaches and nectarines displaying typical brown rot symptoms were collected from multiple provinces in China. A subsample of 26 single-spore isolates were characterized phylogenetically and morphologically to ascertain species. Phylogenetic analysis of internal transcribed spacer (ITS) regions 1 and 2, glyceraldehyde-3-phosphate dehydrogenase (G3PDH), β-tubulin (TUB2) revealed the presence of three distinct Monilinia species. These species included Monilinia fructicola, Monilia mumecola, and a previously undescribed species designated Monilia yunnanensis sp. nov. While M. fructicola is a well-documented pathogen of Prunus persica in China, M. mumecola had primarily only been isolated from mume fruit in Japan. Koch's postulates for M. mumecola and M. yunnanensis were fulfilled confirming pathogenicity of the two species on peach. Phylogenetic analysis of ITS, G3PDH, and TUB2 sequences indicated that M. yunnanensis is most closely related to M. fructigena, a species widely prevalent in Europe. Interestingly, there were considerable differences in the exon/intron structure of the cytochrome b (Cyt b) gene between the two species. Morphological characteristics, including spore size, colony morphology, lesion growth rate, and sporulation, support the phylogenetic evidence suggesting the designation of M. yunnanensis as a new species. A new multiplex PCR method was developed to facilitate the detection of M. yunnanensis and differentiation of Monilinia spp. causing brown rot of peach in China. Public Library of Science 2011-09-27 /pmc/articles/PMC3181254/ /pubmed/21980371 http://dx.doi.org/10.1371/journal.pone.0024990 Text en Hu et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hu, Meng-Jun
Cox, Kerik D.
Schnabel, Guido
Luo, Chao-Xi
Monilinia Species Causing Brown Rot of Peach in China
title Monilinia Species Causing Brown Rot of Peach in China
title_full Monilinia Species Causing Brown Rot of Peach in China
title_fullStr Monilinia Species Causing Brown Rot of Peach in China
title_full_unstemmed Monilinia Species Causing Brown Rot of Peach in China
title_short Monilinia Species Causing Brown Rot of Peach in China
title_sort monilinia species causing brown rot of peach in china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3181254/
https://www.ncbi.nlm.nih.gov/pubmed/21980371
http://dx.doi.org/10.1371/journal.pone.0024990
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