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Identification and Characterization of a Novel Multipotent Sub-Population of Sca-1(+) Cardiac Progenitor Cells for Myocardial Regeneration

METHODS AND RESULTS: The cardiac stem/progenitor cells from adult mice were seeded at low density in serum-free medium. The colonies thus obtained were expanded separately and assessed for expression of stem cell antigen-1 (Sca-1). Two colonies each with high Sca-1 (CSH1; 95.9%; CSH2; 90.6%) and low...

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Autores principales: Takamiya, Michitaka, Haider, Khawaja H., Ashraf, Muhammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3182214/
https://www.ncbi.nlm.nih.gov/pubmed/21980409
http://dx.doi.org/10.1371/journal.pone.0025265
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author Takamiya, Michitaka
Haider, Khawaja H.
Ashraf, Muhammad
author_facet Takamiya, Michitaka
Haider, Khawaja H.
Ashraf, Muhammad
author_sort Takamiya, Michitaka
collection PubMed
description METHODS AND RESULTS: The cardiac stem/progenitor cells from adult mice were seeded at low density in serum-free medium. The colonies thus obtained were expanded separately and assessed for expression of stem cell antigen-1 (Sca-1). Two colonies each with high Sca-1 (CSH1; 95.9%; CSH2; 90.6%) and low Sca-1 (CSL1; 37.1%; CSL2; 17.4%) expressing cells were selected for further studies. Sca-1(+) cells (98.4%) isolated using Magnetic Cell Sorting System (MACS) from the hearts were used as a control. Although the selected populations were similar in surface marker expression (low in c-kit, CD45, CD34, CD31 and high in CD29), these cells exhibited diverse differentiation potential. Unlike CSH1, CSH2 expressed Nanog, TERT, Bcrp1, Nestin, Musashi1 and Isl-1, and also showed differentiation into osteogenic, chondrogenic, smooth muscle, endothelial and cardiac lineages. MACS sorted cells exhibited similar tendency albeit with relatively weaker differentiation potential. Transplantation of CSH2 cells into infarcted heart showed attenuated infarction size, significantly preserved left ventricular function and anterior wall thickness, and increased capillary density. We also observed direct differentiation of transplanted cells into endothelium and cardiomyocytes. CONCLUSIONS: The cardiac stem/progenitor cells isolated by a combined clonal selection and surface marker approach possessed multiple stem cell features important for cardiac regeneration.
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spelling pubmed-31822142011-10-06 Identification and Characterization of a Novel Multipotent Sub-Population of Sca-1(+) Cardiac Progenitor Cells for Myocardial Regeneration Takamiya, Michitaka Haider, Khawaja H. Ashraf, Muhammad PLoS One Research Article METHODS AND RESULTS: The cardiac stem/progenitor cells from adult mice were seeded at low density in serum-free medium. The colonies thus obtained were expanded separately and assessed for expression of stem cell antigen-1 (Sca-1). Two colonies each with high Sca-1 (CSH1; 95.9%; CSH2; 90.6%) and low Sca-1 (CSL1; 37.1%; CSL2; 17.4%) expressing cells were selected for further studies. Sca-1(+) cells (98.4%) isolated using Magnetic Cell Sorting System (MACS) from the hearts were used as a control. Although the selected populations were similar in surface marker expression (low in c-kit, CD45, CD34, CD31 and high in CD29), these cells exhibited diverse differentiation potential. Unlike CSH1, CSH2 expressed Nanog, TERT, Bcrp1, Nestin, Musashi1 and Isl-1, and also showed differentiation into osteogenic, chondrogenic, smooth muscle, endothelial and cardiac lineages. MACS sorted cells exhibited similar tendency albeit with relatively weaker differentiation potential. Transplantation of CSH2 cells into infarcted heart showed attenuated infarction size, significantly preserved left ventricular function and anterior wall thickness, and increased capillary density. We also observed direct differentiation of transplanted cells into endothelium and cardiomyocytes. CONCLUSIONS: The cardiac stem/progenitor cells isolated by a combined clonal selection and surface marker approach possessed multiple stem cell features important for cardiac regeneration. Public Library of Science 2011-09-28 /pmc/articles/PMC3182214/ /pubmed/21980409 http://dx.doi.org/10.1371/journal.pone.0025265 Text en Takamiya et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Takamiya, Michitaka
Haider, Khawaja H.
Ashraf, Muhammad
Identification and Characterization of a Novel Multipotent Sub-Population of Sca-1(+) Cardiac Progenitor Cells for Myocardial Regeneration
title Identification and Characterization of a Novel Multipotent Sub-Population of Sca-1(+) Cardiac Progenitor Cells for Myocardial Regeneration
title_full Identification and Characterization of a Novel Multipotent Sub-Population of Sca-1(+) Cardiac Progenitor Cells for Myocardial Regeneration
title_fullStr Identification and Characterization of a Novel Multipotent Sub-Population of Sca-1(+) Cardiac Progenitor Cells for Myocardial Regeneration
title_full_unstemmed Identification and Characterization of a Novel Multipotent Sub-Population of Sca-1(+) Cardiac Progenitor Cells for Myocardial Regeneration
title_short Identification and Characterization of a Novel Multipotent Sub-Population of Sca-1(+) Cardiac Progenitor Cells for Myocardial Regeneration
title_sort identification and characterization of a novel multipotent sub-population of sca-1(+) cardiac progenitor cells for myocardial regeneration
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3182214/
https://www.ncbi.nlm.nih.gov/pubmed/21980409
http://dx.doi.org/10.1371/journal.pone.0025265
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