Evaluation of Pulsed-FRAP and Conventional-FRAP for Determination of Protein Mobility in Prokaryotic Cells

BACKGROUND: Macromolecule mobility is often quantified with Fluorescence Recovery After Photobleaching (FRAP). Throughout literature a wide range of diffusion coefficients for GFP in the cytoplasm of Escherichia coli (3 to 14 µm(2)/s) is reported using FRAP-based approaches. In this study, we have e...

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Detalles Bibliográficos
Autores principales: Mika, Jacek T., Krasnikov, Victor, van den Bogaart, Geert, de Haan, Foppe, Poolman, Bert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3182251/
https://www.ncbi.nlm.nih.gov/pubmed/21980523
http://dx.doi.org/10.1371/journal.pone.0025664

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