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Sleep-Deprivation Induces Changes in GABA(B) and mGlu Receptor Expression and Has Consequences for Synaptic Long-Term Depression
Long term depression (LTD) in the CA1 region of the hippocampus, induced with a 20-Hz, 30 s tetanus to Schaffer collaterals, is enhanced in sleep-deprived (SD) rats. In the present study, we investigated the role of metabotropic glutamate receptors (mGluRs), γ-Aminobutyric acid (GABA) B receptors (G...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3182263/ https://www.ncbi.nlm.nih.gov/pubmed/21980366 http://dx.doi.org/10.1371/journal.pone.0024933 |
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author | Tadavarty, Ramakrishna Rajput, Padmesh S. Wong, Jennifer M. Kumar, Ujendra Sastry, Bhagavatula R. |
author_facet | Tadavarty, Ramakrishna Rajput, Padmesh S. Wong, Jennifer M. Kumar, Ujendra Sastry, Bhagavatula R. |
author_sort | Tadavarty, Ramakrishna |
collection | PubMed |
description | Long term depression (LTD) in the CA1 region of the hippocampus, induced with a 20-Hz, 30 s tetanus to Schaffer collaterals, is enhanced in sleep-deprived (SD) rats. In the present study, we investigated the role of metabotropic glutamate receptors (mGluRs), γ-Aminobutyric acid (GABA) B receptors (GABA(B)-Rs) and N-methyl-D-aspartic acid receptors (NMDARs) in the LTD of the population excitatory postsynaptic potential (pEPSP). The requirement of Ca(2+) from L- and T- type voltage-gated calcium channels (VGCCs) and intracellular stores was also studied. Results indicate that mGluRs, a release of Ca(2+) from intracellular stores and GABA(B)-Rs are required for LTD. Interestingly, while mGlu1Rs seem to be involved in both short-term depression and LTD, mGlu5Rs appear to participate mostly in LTD. CGP 55845, a GABA(B)-R antagonist, partially suppressed LTD in normally sleeping (NS) rats, while completely blocking LTD in SD rats. Moreover, GS-39783, a positive allosteric modulator for GABA(B)-R, suppressed the pEPSP in SD, but not NS rats. Since both mGluRs and GABA(B)-Rs seem to be involved in the LTD, especially in SD rats, we examined if the receptor expression pattern and/or dimerization changed, using immunohistochemical, co-localization and co-immunoprecipitation techniques. Sleep-deprivation induced an increase in the expression of GABA(B)-R1 and mGlu1αR in the CA1 region of the hippocampus. In addition, co-localization and heterodimerization between mGlu1αR/GABA(B)-R1 and mGlu1αR/GABA(B)-R2 is enhanced in SD rats. Taken together, our findings present a novel form of LTD sensitive to the activation of mGluRs and GABA(B)-Rs, and reveal, for the first time, that sleep-deprivation induces alterations in the expression and dimerization of these receptors. |
format | Online Article Text |
id | pubmed-3182263 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-31822632011-10-06 Sleep-Deprivation Induces Changes in GABA(B) and mGlu Receptor Expression and Has Consequences for Synaptic Long-Term Depression Tadavarty, Ramakrishna Rajput, Padmesh S. Wong, Jennifer M. Kumar, Ujendra Sastry, Bhagavatula R. PLoS One Research Article Long term depression (LTD) in the CA1 region of the hippocampus, induced with a 20-Hz, 30 s tetanus to Schaffer collaterals, is enhanced in sleep-deprived (SD) rats. In the present study, we investigated the role of metabotropic glutamate receptors (mGluRs), γ-Aminobutyric acid (GABA) B receptors (GABA(B)-Rs) and N-methyl-D-aspartic acid receptors (NMDARs) in the LTD of the population excitatory postsynaptic potential (pEPSP). The requirement of Ca(2+) from L- and T- type voltage-gated calcium channels (VGCCs) and intracellular stores was also studied. Results indicate that mGluRs, a release of Ca(2+) from intracellular stores and GABA(B)-Rs are required for LTD. Interestingly, while mGlu1Rs seem to be involved in both short-term depression and LTD, mGlu5Rs appear to participate mostly in LTD. CGP 55845, a GABA(B)-R antagonist, partially suppressed LTD in normally sleeping (NS) rats, while completely blocking LTD in SD rats. Moreover, GS-39783, a positive allosteric modulator for GABA(B)-R, suppressed the pEPSP in SD, but not NS rats. Since both mGluRs and GABA(B)-Rs seem to be involved in the LTD, especially in SD rats, we examined if the receptor expression pattern and/or dimerization changed, using immunohistochemical, co-localization and co-immunoprecipitation techniques. Sleep-deprivation induced an increase in the expression of GABA(B)-R1 and mGlu1αR in the CA1 region of the hippocampus. In addition, co-localization and heterodimerization between mGlu1αR/GABA(B)-R1 and mGlu1αR/GABA(B)-R2 is enhanced in SD rats. Taken together, our findings present a novel form of LTD sensitive to the activation of mGluRs and GABA(B)-Rs, and reveal, for the first time, that sleep-deprivation induces alterations in the expression and dimerization of these receptors. Public Library of Science 2011-09-27 /pmc/articles/PMC3182263/ /pubmed/21980366 http://dx.doi.org/10.1371/journal.pone.0024933 Text en Tadavarty et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Tadavarty, Ramakrishna Rajput, Padmesh S. Wong, Jennifer M. Kumar, Ujendra Sastry, Bhagavatula R. Sleep-Deprivation Induces Changes in GABA(B) and mGlu Receptor Expression and Has Consequences for Synaptic Long-Term Depression |
title | Sleep-Deprivation Induces Changes in GABA(B) and mGlu Receptor Expression and Has Consequences for Synaptic Long-Term Depression |
title_full | Sleep-Deprivation Induces Changes in GABA(B) and mGlu Receptor Expression and Has Consequences for Synaptic Long-Term Depression |
title_fullStr | Sleep-Deprivation Induces Changes in GABA(B) and mGlu Receptor Expression and Has Consequences for Synaptic Long-Term Depression |
title_full_unstemmed | Sleep-Deprivation Induces Changes in GABA(B) and mGlu Receptor Expression and Has Consequences for Synaptic Long-Term Depression |
title_short | Sleep-Deprivation Induces Changes in GABA(B) and mGlu Receptor Expression and Has Consequences for Synaptic Long-Term Depression |
title_sort | sleep-deprivation induces changes in gaba(b) and mglu receptor expression and has consequences for synaptic long-term depression |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3182263/ https://www.ncbi.nlm.nih.gov/pubmed/21980366 http://dx.doi.org/10.1371/journal.pone.0024933 |
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