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Rapid and Efficient Clearance of Blood-borne Virus by Liver Sinusoidal Endothelium

The liver removes quickly the great bulk of virus circulating in blood, leaving only a small fraction to infect the host, in a manner characteristic of each virus. The scavenger cells of the liver sinusoids are implicated, but the mechanism is entirely unknown. Here we show, borrowing a mouse model...

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Autores principales: Ganesan, Latha P., Mohanty, Sudhasri, Kim, Jonghan, Clark, K. Reed, Robinson, John M., Anderson, Clark L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3182912/
https://www.ncbi.nlm.nih.gov/pubmed/21980295
http://dx.doi.org/10.1371/journal.ppat.1002281
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author Ganesan, Latha P.
Mohanty, Sudhasri
Kim, Jonghan
Clark, K. Reed
Robinson, John M.
Anderson, Clark L.
author_facet Ganesan, Latha P.
Mohanty, Sudhasri
Kim, Jonghan
Clark, K. Reed
Robinson, John M.
Anderson, Clark L.
author_sort Ganesan, Latha P.
collection PubMed
description The liver removes quickly the great bulk of virus circulating in blood, leaving only a small fraction to infect the host, in a manner characteristic of each virus. The scavenger cells of the liver sinusoids are implicated, but the mechanism is entirely unknown. Here we show, borrowing a mouse model of adenovirus clearance, that nearly all infused adenovirus is cleared by the liver sinusoidal endothelial cell (LSEC). Using refined immunofluorescence microscopy techniques for distinguishing macrophages and endothelial cells in fixed liver, and identifying virus by two distinct physicochemical methods, we localized adenovirus 1 minute after infusion mainly to the LSEC (∼90%), finding ∼10% with Kupffer cells (KC) and none with hepatocytes. Electron microscopy confirmed our results. In contrast with much prior work claiming the main scavenger to be the KC, our results locate the clearance mechanism to the LSEC and identify this cell as a key site of antiviral activity.
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spelling pubmed-31829122011-10-06 Rapid and Efficient Clearance of Blood-borne Virus by Liver Sinusoidal Endothelium Ganesan, Latha P. Mohanty, Sudhasri Kim, Jonghan Clark, K. Reed Robinson, John M. Anderson, Clark L. PLoS Pathog Research Article The liver removes quickly the great bulk of virus circulating in blood, leaving only a small fraction to infect the host, in a manner characteristic of each virus. The scavenger cells of the liver sinusoids are implicated, but the mechanism is entirely unknown. Here we show, borrowing a mouse model of adenovirus clearance, that nearly all infused adenovirus is cleared by the liver sinusoidal endothelial cell (LSEC). Using refined immunofluorescence microscopy techniques for distinguishing macrophages and endothelial cells in fixed liver, and identifying virus by two distinct physicochemical methods, we localized adenovirus 1 minute after infusion mainly to the LSEC (∼90%), finding ∼10% with Kupffer cells (KC) and none with hepatocytes. Electron microscopy confirmed our results. In contrast with much prior work claiming the main scavenger to be the KC, our results locate the clearance mechanism to the LSEC and identify this cell as a key site of antiviral activity. Public Library of Science 2011-09-29 /pmc/articles/PMC3182912/ /pubmed/21980295 http://dx.doi.org/10.1371/journal.ppat.1002281 Text en Ganesan et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ganesan, Latha P.
Mohanty, Sudhasri
Kim, Jonghan
Clark, K. Reed
Robinson, John M.
Anderson, Clark L.
Rapid and Efficient Clearance of Blood-borne Virus by Liver Sinusoidal Endothelium
title Rapid and Efficient Clearance of Blood-borne Virus by Liver Sinusoidal Endothelium
title_full Rapid and Efficient Clearance of Blood-borne Virus by Liver Sinusoidal Endothelium
title_fullStr Rapid and Efficient Clearance of Blood-borne Virus by Liver Sinusoidal Endothelium
title_full_unstemmed Rapid and Efficient Clearance of Blood-borne Virus by Liver Sinusoidal Endothelium
title_short Rapid and Efficient Clearance of Blood-borne Virus by Liver Sinusoidal Endothelium
title_sort rapid and efficient clearance of blood-borne virus by liver sinusoidal endothelium
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3182912/
https://www.ncbi.nlm.nih.gov/pubmed/21980295
http://dx.doi.org/10.1371/journal.ppat.1002281
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