Improved protein arrays for quantitative systems analysis of the dynamics of signaling pathway interactions

An improved version of quantitative protein array platform utilizing linear Quantum dot signaling for systematically measuring protein levels and phosphorylation states is presented. The signals are amplified linearly by a confocal laser Quantum dot scanner resulting in ~1000-fold more sensitivity t...

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Detalles Bibliográficos
Autores principales: Wang, Xiaoyu, Dong, Ying, Jiwani, Ameena J, Zou, Yonglong, Pastor, Johanne, Kuro-o, Makoto, Habib, Amyn A, Ruan, Minzi, Boothman, David A, Yang, Chin-Rang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3182966/
https://www.ncbi.nlm.nih.gov/pubmed/21917185
http://dx.doi.org/10.1186/1477-5956-9-53
Descripción
Sumario:An improved version of quantitative protein array platform utilizing linear Quantum dot signaling for systematically measuring protein levels and phosphorylation states is presented. The signals are amplified linearly by a confocal laser Quantum dot scanner resulting in ~1000-fold more sensitivity than traditional Western blots, but are not linear by the enzyme-based amplification. Software is developed to facilitate the quantitative readouts of signaling network activities. Kinetics of EGFRvIII mutant signaling was analyzed to quantify cross-talks between EGFR and other signaling pathways.

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