Improved protein arrays for quantitative systems analysis of the dynamics of signaling pathway interactions

An improved version of quantitative protein array platform utilizing linear Quantum dot signaling for systematically measuring protein levels and phosphorylation states is presented. The signals are amplified linearly by a confocal laser Quantum dot scanner resulting in ~1000-fold more sensitivity t...

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Autores principales: Wang, Xiaoyu, Dong, Ying, Jiwani, Ameena J, Zou, Yonglong, Pastor, Johanne, Kuro-o, Makoto, Habib, Amyn A, Ruan, Minzi, Boothman, David A, Yang, Chin-Rang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3182966/
https://www.ncbi.nlm.nih.gov/pubmed/21917185
http://dx.doi.org/10.1186/1477-5956-9-53
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author Wang, Xiaoyu
Dong, Ying
Jiwani, Ameena J
Zou, Yonglong
Pastor, Johanne
Kuro-o, Makoto
Habib, Amyn A
Ruan, Minzi
Boothman, David A
Yang, Chin-Rang
author_facet Wang, Xiaoyu
Dong, Ying
Jiwani, Ameena J
Zou, Yonglong
Pastor, Johanne
Kuro-o, Makoto
Habib, Amyn A
Ruan, Minzi
Boothman, David A
Yang, Chin-Rang
author_sort Wang, Xiaoyu
collection PubMed
description An improved version of quantitative protein array platform utilizing linear Quantum dot signaling for systematically measuring protein levels and phosphorylation states is presented. The signals are amplified linearly by a confocal laser Quantum dot scanner resulting in ~1000-fold more sensitivity than traditional Western blots, but are not linear by the enzyme-based amplification. Software is developed to facilitate the quantitative readouts of signaling network activities. Kinetics of EGFRvIII mutant signaling was analyzed to quantify cross-talks between EGFR and other signaling pathways.
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spelling pubmed-31829662011-09-30 Improved protein arrays for quantitative systems analysis of the dynamics of signaling pathway interactions Wang, Xiaoyu Dong, Ying Jiwani, Ameena J Zou, Yonglong Pastor, Johanne Kuro-o, Makoto Habib, Amyn A Ruan, Minzi Boothman, David A Yang, Chin-Rang Proteome Sci Methodology An improved version of quantitative protein array platform utilizing linear Quantum dot signaling for systematically measuring protein levels and phosphorylation states is presented. The signals are amplified linearly by a confocal laser Quantum dot scanner resulting in ~1000-fold more sensitivity than traditional Western blots, but are not linear by the enzyme-based amplification. Software is developed to facilitate the quantitative readouts of signaling network activities. Kinetics of EGFRvIII mutant signaling was analyzed to quantify cross-talks between EGFR and other signaling pathways. BioMed Central 2011-09-15 /pmc/articles/PMC3182966/ /pubmed/21917185 http://dx.doi.org/10.1186/1477-5956-9-53 Text en Copyright ©2011 Wang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
Wang, Xiaoyu
Dong, Ying
Jiwani, Ameena J
Zou, Yonglong
Pastor, Johanne
Kuro-o, Makoto
Habib, Amyn A
Ruan, Minzi
Boothman, David A
Yang, Chin-Rang
Improved protein arrays for quantitative systems analysis of the dynamics of signaling pathway interactions
title Improved protein arrays for quantitative systems analysis of the dynamics of signaling pathway interactions
title_full Improved protein arrays for quantitative systems analysis of the dynamics of signaling pathway interactions
title_fullStr Improved protein arrays for quantitative systems analysis of the dynamics of signaling pathway interactions
title_full_unstemmed Improved protein arrays for quantitative systems analysis of the dynamics of signaling pathway interactions
title_short Improved protein arrays for quantitative systems analysis of the dynamics of signaling pathway interactions
title_sort improved protein arrays for quantitative systems analysis of the dynamics of signaling pathway interactions
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3182966/
https://www.ncbi.nlm.nih.gov/pubmed/21917185
http://dx.doi.org/10.1186/1477-5956-9-53
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