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Kinesin-3 and dynein cooperate in long-range retrograde endosome motility along a nonuniform microtubule array
The polarity of microtubules (MTs) determines the motors for intracellular motility, with kinesins moving to plus ends and dynein to minus ends. In elongated cells of Ustilago maydis, dynein is thought to move early endosomes (EEs) toward the septum (retrograde), whereas kinesin-3 transports them to...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3183019/ https://www.ncbi.nlm.nih.gov/pubmed/21832152 http://dx.doi.org/10.1091/mbc.E11-03-0217 |
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author | Schuster, Martin Kilaru, Sreedhar Fink, Gero Collemare, Jérôme Roger, Yvonne Steinberg, Gero |
author_facet | Schuster, Martin Kilaru, Sreedhar Fink, Gero Collemare, Jérôme Roger, Yvonne Steinberg, Gero |
author_sort | Schuster, Martin |
collection | PubMed |
description | The polarity of microtubules (MTs) determines the motors for intracellular motility, with kinesins moving to plus ends and dynein to minus ends. In elongated cells of Ustilago maydis, dynein is thought to move early endosomes (EEs) toward the septum (retrograde), whereas kinesin-3 transports them to the growing cell tip (anterograde). Occasionally, EEs run up to 90 μm in one direction. The underlying MT array consists of unipolar MTs at both cell ends and antipolar bundles in the middle region of the cell. Cytoplasmic MT-organizing centers, labeled with a γ-tubulin ring complex protein, are distributed along the antipolar MTs but are absent from the unipolar regions. Dynein colocalizes with EEs for 10–20 μm after they have left the cell tip. Inactivation of temperature-sensitive dynein abolishes EE motility within the unipolar MT array, whereas long-range motility is not impaired. In contrast, kinesin-3 is continuously present, and its inactivation stops long-range EE motility. This indicates that both motors participate in EE motility, with dynein transporting the organelles through the unipolar MT array near the cell ends, and kinesin-3 taking over at the beginning of the medial antipolar MT array. The cooperation of both motors mediates EE movements over the length of the entire cell. |
format | Online Article Text |
id | pubmed-3183019 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-31830192011-12-16 Kinesin-3 and dynein cooperate in long-range retrograde endosome motility along a nonuniform microtubule array Schuster, Martin Kilaru, Sreedhar Fink, Gero Collemare, Jérôme Roger, Yvonne Steinberg, Gero Mol Biol Cell Articles The polarity of microtubules (MTs) determines the motors for intracellular motility, with kinesins moving to plus ends and dynein to minus ends. In elongated cells of Ustilago maydis, dynein is thought to move early endosomes (EEs) toward the septum (retrograde), whereas kinesin-3 transports them to the growing cell tip (anterograde). Occasionally, EEs run up to 90 μm in one direction. The underlying MT array consists of unipolar MTs at both cell ends and antipolar bundles in the middle region of the cell. Cytoplasmic MT-organizing centers, labeled with a γ-tubulin ring complex protein, are distributed along the antipolar MTs but are absent from the unipolar regions. Dynein colocalizes with EEs for 10–20 μm after they have left the cell tip. Inactivation of temperature-sensitive dynein abolishes EE motility within the unipolar MT array, whereas long-range motility is not impaired. In contrast, kinesin-3 is continuously present, and its inactivation stops long-range EE motility. This indicates that both motors participate in EE motility, with dynein transporting the organelles through the unipolar MT array near the cell ends, and kinesin-3 taking over at the beginning of the medial antipolar MT array. The cooperation of both motors mediates EE movements over the length of the entire cell. The American Society for Cell Biology 2011-10-01 /pmc/articles/PMC3183019/ /pubmed/21832152 http://dx.doi.org/10.1091/mbc.E11-03-0217 Text en © 2011 Schuster et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology. |
spellingShingle | Articles Schuster, Martin Kilaru, Sreedhar Fink, Gero Collemare, Jérôme Roger, Yvonne Steinberg, Gero Kinesin-3 and dynein cooperate in long-range retrograde endosome motility along a nonuniform microtubule array |
title | Kinesin-3 and dynein cooperate in long-range retrograde endosome motility along a nonuniform microtubule array |
title_full | Kinesin-3 and dynein cooperate in long-range retrograde endosome motility along a nonuniform microtubule array |
title_fullStr | Kinesin-3 and dynein cooperate in long-range retrograde endosome motility along a nonuniform microtubule array |
title_full_unstemmed | Kinesin-3 and dynein cooperate in long-range retrograde endosome motility along a nonuniform microtubule array |
title_short | Kinesin-3 and dynein cooperate in long-range retrograde endosome motility along a nonuniform microtubule array |
title_sort | kinesin-3 and dynein cooperate in long-range retrograde endosome motility along a nonuniform microtubule array |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3183019/ https://www.ncbi.nlm.nih.gov/pubmed/21832152 http://dx.doi.org/10.1091/mbc.E11-03-0217 |
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