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Endocytosis is essential for dynamic translocation of a syntaxin 1 orthologue during fission yeast meiosis

Syntaxin is a component of the target soluble N-ethylmaleimide–sensitive factor attachment protein receptor complex, which is responsible for fusion of membrane vesicles at the target membrane. The fission yeast syntaxin 1 orthologue Psy1 is essential for both vegetative growth and spore formation....

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Autores principales: Kashiwazaki, Jun, Yamasaki, Yuriko, Itadani, Akiko, Teraguchi, Erika, Maeda, Yukari, Shimoda, Chikashi, Nakamura, Taro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3183020/
https://www.ncbi.nlm.nih.gov/pubmed/21832151
http://dx.doi.org/10.1091/mbc.E11-03-0255
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author Kashiwazaki, Jun
Yamasaki, Yuriko
Itadani, Akiko
Teraguchi, Erika
Maeda, Yukari
Shimoda, Chikashi
Nakamura, Taro
author_facet Kashiwazaki, Jun
Yamasaki, Yuriko
Itadani, Akiko
Teraguchi, Erika
Maeda, Yukari
Shimoda, Chikashi
Nakamura, Taro
author_sort Kashiwazaki, Jun
collection PubMed
description Syntaxin is a component of the target soluble N-ethylmaleimide–sensitive factor attachment protein receptor complex, which is responsible for fusion of membrane vesicles at the target membrane. The fission yeast syntaxin 1 orthologue Psy1 is essential for both vegetative growth and spore formation. During meiosis, Psy1 disappears from the plasma membrane (PM) and dramatically relocalizes on the nascent forespore membrane, which becomes the PM of the spore. Here we report the molecular details and biological significance of Psy1 relocalization. We find that, immediately after meiosis I, Psy1 is selectively internalized by endocytosis. In addition, a meiosis-specific signal induced by the transcription factor Mei4 seems to trigger this internalization. The internalization of many PM proteins is facilitated coincident with the initiation of meiosis, whereas Pma1, a P-type ATPase, persists on the PM even during the progression of meiosis II. Ergosterol on the PM is also important for the internalization of PM proteins in general during meiosis. We consider that during meiosis in Schizosaccharomyces pombe cells, the characteristics of endocytosis change, thereby facilitating internalization of Psy1 and accomplishing sporulation.
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spelling pubmed-31830202011-12-16 Endocytosis is essential for dynamic translocation of a syntaxin 1 orthologue during fission yeast meiosis Kashiwazaki, Jun Yamasaki, Yuriko Itadani, Akiko Teraguchi, Erika Maeda, Yukari Shimoda, Chikashi Nakamura, Taro Mol Biol Cell Articles Syntaxin is a component of the target soluble N-ethylmaleimide–sensitive factor attachment protein receptor complex, which is responsible for fusion of membrane vesicles at the target membrane. The fission yeast syntaxin 1 orthologue Psy1 is essential for both vegetative growth and spore formation. During meiosis, Psy1 disappears from the plasma membrane (PM) and dramatically relocalizes on the nascent forespore membrane, which becomes the PM of the spore. Here we report the molecular details and biological significance of Psy1 relocalization. We find that, immediately after meiosis I, Psy1 is selectively internalized by endocytosis. In addition, a meiosis-specific signal induced by the transcription factor Mei4 seems to trigger this internalization. The internalization of many PM proteins is facilitated coincident with the initiation of meiosis, whereas Pma1, a P-type ATPase, persists on the PM even during the progression of meiosis II. Ergosterol on the PM is also important for the internalization of PM proteins in general during meiosis. We consider that during meiosis in Schizosaccharomyces pombe cells, the characteristics of endocytosis change, thereby facilitating internalization of Psy1 and accomplishing sporulation. The American Society for Cell Biology 2011-10-01 /pmc/articles/PMC3183020/ /pubmed/21832151 http://dx.doi.org/10.1091/mbc.E11-03-0255 Text en © 2011 Kashiwazaki et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Kashiwazaki, Jun
Yamasaki, Yuriko
Itadani, Akiko
Teraguchi, Erika
Maeda, Yukari
Shimoda, Chikashi
Nakamura, Taro
Endocytosis is essential for dynamic translocation of a syntaxin 1 orthologue during fission yeast meiosis
title Endocytosis is essential for dynamic translocation of a syntaxin 1 orthologue during fission yeast meiosis
title_full Endocytosis is essential for dynamic translocation of a syntaxin 1 orthologue during fission yeast meiosis
title_fullStr Endocytosis is essential for dynamic translocation of a syntaxin 1 orthologue during fission yeast meiosis
title_full_unstemmed Endocytosis is essential for dynamic translocation of a syntaxin 1 orthologue during fission yeast meiosis
title_short Endocytosis is essential for dynamic translocation of a syntaxin 1 orthologue during fission yeast meiosis
title_sort endocytosis is essential for dynamic translocation of a syntaxin 1 orthologue during fission yeast meiosis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3183020/
https://www.ncbi.nlm.nih.gov/pubmed/21832151
http://dx.doi.org/10.1091/mbc.E11-03-0255
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