Survival of Burkholderia pseudomallei in distilled water for 16 years

Burkholderia pseudomallei was examined after being maintained in distilled water at 25 °C for 16 years. The Gram stain was atypical (pale pink cocci or coccobacilli). The estimated number of live and dead B. pseudomallei was 3.8 × 10(7) cells/ml and 1.4 × 10(5) cells/ml, respectively. A colony count...

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Detalles Bibliográficos
Autores principales: Pumpuang, Apinya, Chantratita, Narisara, Wikraiphat, Chanthiwa, Saiprom, Natnaree, Day, Nicholas P.J., Peacock, Sharon J., Wuthiekanun, Vanaporn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3183224/
https://www.ncbi.nlm.nih.gov/pubmed/21764093
http://dx.doi.org/10.1016/j.trstmh.2011.06.004
Descripción
Sumario:Burkholderia pseudomallei was examined after being maintained in distilled water at 25 °C for 16 years. The Gram stain was atypical (pale pink cocci or coccobacilli). The estimated number of live and dead B. pseudomallei was 3.8 × 10(7) cells/ml and 1.4 × 10(5) cells/ml, respectively. A colony count on agar of 1.0 × 10(6) cfu/ml suggested that a proportion of cells were in a viable but non-culturable state. Colony morphology was different from the parental isolate for 84% of colonies. Pulsed-field gel electrophoresis analysis of AvrII DNA restriction fragments revealed six different but related banding patterns, which may represent genomic rearrangement.

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