Survival of Burkholderia pseudomallei in distilled water for 16 years

Burkholderia pseudomallei was examined after being maintained in distilled water at 25 °C for 16 years. The Gram stain was atypical (pale pink cocci or coccobacilli). The estimated number of live and dead B. pseudomallei was 3.8 × 10(7) cells/ml and 1.4 × 10(5) cells/ml, respectively. A colony count...

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Autores principales: Pumpuang, Apinya, Chantratita, Narisara, Wikraiphat, Chanthiwa, Saiprom, Natnaree, Day, Nicholas P.J., Peacock, Sharon J., Wuthiekanun, Vanaporn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3183224/
https://www.ncbi.nlm.nih.gov/pubmed/21764093
http://dx.doi.org/10.1016/j.trstmh.2011.06.004
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author Pumpuang, Apinya
Chantratita, Narisara
Wikraiphat, Chanthiwa
Saiprom, Natnaree
Day, Nicholas P.J.
Peacock, Sharon J.
Wuthiekanun, Vanaporn
author_facet Pumpuang, Apinya
Chantratita, Narisara
Wikraiphat, Chanthiwa
Saiprom, Natnaree
Day, Nicholas P.J.
Peacock, Sharon J.
Wuthiekanun, Vanaporn
author_sort Pumpuang, Apinya
collection PubMed
description Burkholderia pseudomallei was examined after being maintained in distilled water at 25 °C for 16 years. The Gram stain was atypical (pale pink cocci or coccobacilli). The estimated number of live and dead B. pseudomallei was 3.8 × 10(7) cells/ml and 1.4 × 10(5) cells/ml, respectively. A colony count on agar of 1.0 × 10(6) cfu/ml suggested that a proportion of cells were in a viable but non-culturable state. Colony morphology was different from the parental isolate for 84% of colonies. Pulsed-field gel electrophoresis analysis of AvrII DNA restriction fragments revealed six different but related banding patterns, which may represent genomic rearrangement.
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spelling pubmed-31832242011-10-01 Survival of Burkholderia pseudomallei in distilled water for 16 years Pumpuang, Apinya Chantratita, Narisara Wikraiphat, Chanthiwa Saiprom, Natnaree Day, Nicholas P.J. Peacock, Sharon J. Wuthiekanun, Vanaporn Trans R Soc Trop Med Hyg Short Communication Burkholderia pseudomallei was examined after being maintained in distilled water at 25 °C for 16 years. The Gram stain was atypical (pale pink cocci or coccobacilli). The estimated number of live and dead B. pseudomallei was 3.8 × 10(7) cells/ml and 1.4 × 10(5) cells/ml, respectively. A colony count on agar of 1.0 × 10(6) cfu/ml suggested that a proportion of cells were in a viable but non-culturable state. Colony morphology was different from the parental isolate for 84% of colonies. Pulsed-field gel electrophoresis analysis of AvrII DNA restriction fragments revealed six different but related banding patterns, which may represent genomic rearrangement. Oxford University Press 2011-10 /pmc/articles/PMC3183224/ /pubmed/21764093 http://dx.doi.org/10.1016/j.trstmh.2011.06.004 Text en © 2011 Elsevier Ltd. https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use.
spellingShingle Short Communication
Pumpuang, Apinya
Chantratita, Narisara
Wikraiphat, Chanthiwa
Saiprom, Natnaree
Day, Nicholas P.J.
Peacock, Sharon J.
Wuthiekanun, Vanaporn
Survival of Burkholderia pseudomallei in distilled water for 16 years
title Survival of Burkholderia pseudomallei in distilled water for 16 years
title_full Survival of Burkholderia pseudomallei in distilled water for 16 years
title_fullStr Survival of Burkholderia pseudomallei in distilled water for 16 years
title_full_unstemmed Survival of Burkholderia pseudomallei in distilled water for 16 years
title_short Survival of Burkholderia pseudomallei in distilled water for 16 years
title_sort survival of burkholderia pseudomallei in distilled water for 16 years
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3183224/
https://www.ncbi.nlm.nih.gov/pubmed/21764093
http://dx.doi.org/10.1016/j.trstmh.2011.06.004
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