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In vivo quantification of photosensitizer fluorescence in the skin-fold observation chamber using dual-wavelength excitation and NIR imaging

A major challenge in biomedical optics is the accurate quantification of in vivo fluorescence images. Fluorescence imaging is often used to determine the pharmacokinetics of photosensitizers used for photodynamic therapy. Often, however, this type of imaging does not take into account differences in...

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Autores principales: Kaščáková, Slávka, de Visscher, Sebastiaan, Kruijt, Bastiaan, de Bruijn, Henriëtte S., van der Ploeg-van den Heuvel, Angélique, Sterenborg, Henricus J. C. M., Witjes, Max J. H., Amelink, Arjen, Robinson, Dominic J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3183248/
https://www.ncbi.nlm.nih.gov/pubmed/21279401
http://dx.doi.org/10.1007/s10103-011-0888-z
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author Kaščáková, Slávka
de Visscher, Sebastiaan
Kruijt, Bastiaan
de Bruijn, Henriëtte S.
van der Ploeg-van den Heuvel, Angélique
Sterenborg, Henricus J. C. M.
Witjes, Max J. H.
Amelink, Arjen
Robinson, Dominic J.
author_facet Kaščáková, Slávka
de Visscher, Sebastiaan
Kruijt, Bastiaan
de Bruijn, Henriëtte S.
van der Ploeg-van den Heuvel, Angélique
Sterenborg, Henricus J. C. M.
Witjes, Max J. H.
Amelink, Arjen
Robinson, Dominic J.
author_sort Kaščáková, Slávka
collection PubMed
description A major challenge in biomedical optics is the accurate quantification of in vivo fluorescence images. Fluorescence imaging is often used to determine the pharmacokinetics of photosensitizers used for photodynamic therapy. Often, however, this type of imaging does not take into account differences in and changes to tissue volume and optical properties of the tissue under interrogation. To address this problem, a ratiometric quantification method was developed and applied to monitor photosensitizer meso-tetra(hydroxyphenyl) chlorin (mTHPC) pharmacokinetics in the rat skin-fold observation chamber. The method employs a combination of dual-wavelength excitation and dual-wavelength detection. Excitation and detection wavelengths were selected in the NIR region. One excitation wavelength was chosen to be at the Q band of mTHPC, whereas the second excitation wavelength was close to its absorption minimum. Two fluorescence emission bands were used; one at the secondary fluorescence maximum of mTHPC centered on 720 nm, and one in a region of tissue autofluorescence. The first excitation wavelength was used to excite the mTHPC and autofluorescence and the second to excite only autofluorescence, so that this could be subtracted. Subsequently, the autofluorescence-corrected mTHPC image was divided by the autofluorescence signal to correct for variations in tissue optical properties. This correction algorithm in principle results in a linear relation between the corrected fluorescence and photosensitizer concentration. The limitations of the presented method and comparison with previously published and validated techniques are discussed.
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spelling pubmed-31832482011-10-04 In vivo quantification of photosensitizer fluorescence in the skin-fold observation chamber using dual-wavelength excitation and NIR imaging Kaščáková, Slávka de Visscher, Sebastiaan Kruijt, Bastiaan de Bruijn, Henriëtte S. van der Ploeg-van den Heuvel, Angélique Sterenborg, Henricus J. C. M. Witjes, Max J. H. Amelink, Arjen Robinson, Dominic J. Lasers Med Sci Original Article A major challenge in biomedical optics is the accurate quantification of in vivo fluorescence images. Fluorescence imaging is often used to determine the pharmacokinetics of photosensitizers used for photodynamic therapy. Often, however, this type of imaging does not take into account differences in and changes to tissue volume and optical properties of the tissue under interrogation. To address this problem, a ratiometric quantification method was developed and applied to monitor photosensitizer meso-tetra(hydroxyphenyl) chlorin (mTHPC) pharmacokinetics in the rat skin-fold observation chamber. The method employs a combination of dual-wavelength excitation and dual-wavelength detection. Excitation and detection wavelengths were selected in the NIR region. One excitation wavelength was chosen to be at the Q band of mTHPC, whereas the second excitation wavelength was close to its absorption minimum. Two fluorescence emission bands were used; one at the secondary fluorescence maximum of mTHPC centered on 720 nm, and one in a region of tissue autofluorescence. The first excitation wavelength was used to excite the mTHPC and autofluorescence and the second to excite only autofluorescence, so that this could be subtracted. Subsequently, the autofluorescence-corrected mTHPC image was divided by the autofluorescence signal to correct for variations in tissue optical properties. This correction algorithm in principle results in a linear relation between the corrected fluorescence and photosensitizer concentration. The limitations of the presented method and comparison with previously published and validated techniques are discussed. Springer-Verlag 2011-01-29 2011 /pmc/articles/PMC3183248/ /pubmed/21279401 http://dx.doi.org/10.1007/s10103-011-0888-z Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Original Article
Kaščáková, Slávka
de Visscher, Sebastiaan
Kruijt, Bastiaan
de Bruijn, Henriëtte S.
van der Ploeg-van den Heuvel, Angélique
Sterenborg, Henricus J. C. M.
Witjes, Max J. H.
Amelink, Arjen
Robinson, Dominic J.
In vivo quantification of photosensitizer fluorescence in the skin-fold observation chamber using dual-wavelength excitation and NIR imaging
title In vivo quantification of photosensitizer fluorescence in the skin-fold observation chamber using dual-wavelength excitation and NIR imaging
title_full In vivo quantification of photosensitizer fluorescence in the skin-fold observation chamber using dual-wavelength excitation and NIR imaging
title_fullStr In vivo quantification of photosensitizer fluorescence in the skin-fold observation chamber using dual-wavelength excitation and NIR imaging
title_full_unstemmed In vivo quantification of photosensitizer fluorescence in the skin-fold observation chamber using dual-wavelength excitation and NIR imaging
title_short In vivo quantification of photosensitizer fluorescence in the skin-fold observation chamber using dual-wavelength excitation and NIR imaging
title_sort in vivo quantification of photosensitizer fluorescence in the skin-fold observation chamber using dual-wavelength excitation and nir imaging
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3183248/
https://www.ncbi.nlm.nih.gov/pubmed/21279401
http://dx.doi.org/10.1007/s10103-011-0888-z
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