Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells

BACKGROUND: Identification of secreted proteins of low abundance is often limited by abundant and high molecular weight (MW) proteins. We have optimised a procedure to overcome this limitation. RESULTS: Low MW proteins in the conditioned media of cultured cells were first captured using dual-size ex...

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Detalles Bibliográficos
Autores principales: Paule, Sarah, Meehan, Katie, Rainczuk, Adam, Stephens, Andrew N, Nie, Guiying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184050/
https://www.ncbi.nlm.nih.gov/pubmed/21884602
http://dx.doi.org/10.1186/1477-5956-9-50
Descripción
Sumario:BACKGROUND: Identification of secreted proteins of low abundance is often limited by abundant and high molecular weight (MW) proteins. We have optimised a procedure to overcome this limitation. RESULTS: Low MW proteins in the conditioned media of cultured cells were first captured using dual-size exclusion/affinity hydrogel nanoparticles and their identities were then revealed by proteomics. CONCLUSIONS: This technique enables the analysis of secreted proteins of cultured cells low MW and low abundance.

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