Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells

BACKGROUND: Identification of secreted proteins of low abundance is often limited by abundant and high molecular weight (MW) proteins. We have optimised a procedure to overcome this limitation. RESULTS: Low MW proteins in the conditioned media of cultured cells were first captured using dual-size ex...

Descripción completa

Detalles Bibliográficos
Autores principales: Paule, Sarah, Meehan, Katie, Rainczuk, Adam, Stephens, Andrew N, Nie, Guiying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184050/
https://www.ncbi.nlm.nih.gov/pubmed/21884602
http://dx.doi.org/10.1186/1477-5956-9-50
_version_ 1782213047567253504
author Paule, Sarah
Meehan, Katie
Rainczuk, Adam
Stephens, Andrew N
Nie, Guiying
author_facet Paule, Sarah
Meehan, Katie
Rainczuk, Adam
Stephens, Andrew N
Nie, Guiying
author_sort Paule, Sarah
collection PubMed
description BACKGROUND: Identification of secreted proteins of low abundance is often limited by abundant and high molecular weight (MW) proteins. We have optimised a procedure to overcome this limitation. RESULTS: Low MW proteins in the conditioned media of cultured cells were first captured using dual-size exclusion/affinity hydrogel nanoparticles and their identities were then revealed by proteomics. CONCLUSIONS: This technique enables the analysis of secreted proteins of cultured cells low MW and low abundance.
format Online
Article
Text
id pubmed-3184050
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-31840502011-10-01 Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells Paule, Sarah Meehan, Katie Rainczuk, Adam Stephens, Andrew N Nie, Guiying Proteome Sci Methodology BACKGROUND: Identification of secreted proteins of low abundance is often limited by abundant and high molecular weight (MW) proteins. We have optimised a procedure to overcome this limitation. RESULTS: Low MW proteins in the conditioned media of cultured cells were first captured using dual-size exclusion/affinity hydrogel nanoparticles and their identities were then revealed by proteomics. CONCLUSIONS: This technique enables the analysis of secreted proteins of cultured cells low MW and low abundance. BioMed Central 2011-09-01 /pmc/articles/PMC3184050/ /pubmed/21884602 http://dx.doi.org/10.1186/1477-5956-9-50 Text en Copyright ©2011 Paule et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
Paule, Sarah
Meehan, Katie
Rainczuk, Adam
Stephens, Andrew N
Nie, Guiying
Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells
title Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells
title_full Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells
title_fullStr Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells
title_full_unstemmed Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells
title_short Combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells
title_sort combination of hydrogel nanoparticles and proteomics to reveal secreted proteins associated with decidualization of human uterine stromal cells
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184050/
https://www.ncbi.nlm.nih.gov/pubmed/21884602
http://dx.doi.org/10.1186/1477-5956-9-50
work_keys_str_mv AT paulesarah combinationofhydrogelnanoparticlesandproteomicstorevealsecretedproteinsassociatedwithdecidualizationofhumanuterinestromalcells
AT meehankatie combinationofhydrogelnanoparticlesandproteomicstorevealsecretedproteinsassociatedwithdecidualizationofhumanuterinestromalcells
AT rainczukadam combinationofhydrogelnanoparticlesandproteomicstorevealsecretedproteinsassociatedwithdecidualizationofhumanuterinestromalcells
AT stephensandrewn combinationofhydrogelnanoparticlesandproteomicstorevealsecretedproteinsassociatedwithdecidualizationofhumanuterinestromalcells
AT nieguiying combinationofhydrogelnanoparticlesandproteomicstorevealsecretedproteinsassociatedwithdecidualizationofhumanuterinestromalcells

Ejemplares similares