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Adenoviruses in Lymphocytes of the Human Gastro-Intestinal Tract
OBJECTIVE: Persistent adenoviral shedding in stools is known to occur past convalescence following acute adenoviral infections. We wished to establish the frequency with which adenoviruses may colonize the gut in normal human subjects. METHODS: The presence of adenoviral DNA in intestinal specimens...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184098/ https://www.ncbi.nlm.nih.gov/pubmed/21980361 http://dx.doi.org/10.1371/journal.pone.0024859 |
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author | Roy, Soumitra Calcedo, Roberto Medina-Jaszek, Angelica Keough, Martin Peng, Hui Wilson, James M. |
author_facet | Roy, Soumitra Calcedo, Roberto Medina-Jaszek, Angelica Keough, Martin Peng, Hui Wilson, James M. |
author_sort | Roy, Soumitra |
collection | PubMed |
description | OBJECTIVE: Persistent adenoviral shedding in stools is known to occur past convalescence following acute adenoviral infections. We wished to establish the frequency with which adenoviruses may colonize the gut in normal human subjects. METHODS: The presence of adenoviral DNA in intestinal specimens obtained at surgery or autopsy was tested using a nested PCR method. The amplified adenoviral DNA sequences were compared to each other and to known adenoviral species. Lamina propria lymphocytes (LPLs) were isolated from the specimens and the adenoviral copy numbers in the CD4+ and CD8+ fractions were determined by quantitative PCR. Adenoviral gene expression was tested by amplification of adenoviral mRNA. RESULTS: Intestinal tissue from 21 of 58 donors and LPLs from 21 of 24 donors were positive for the presence of adenoviral DNA. The majority of the sequences could be assigned to adenoviral species E, although species B and C sequences were also common. Multiple sequences were often present in the same sample. Forty-one non-identical sequences were identified from 39 different tissue donors. Quantitative PCR for adenoviral DNA in CD4+ and CD8+ fractions of LPLs showed adenoviral DNA to be present in both cell types and ranged from a few hundred to several million copies per million cells on average. Active adenoviral gene expression as evidenced by the presence of adenoviral messenger RNA in intestinal lymphocytes was demonstrated in 9 of the 11 donors tested. CONCLUSION: Adenoviral DNA is highly prevalent in lymphocytes from the gastro-intestinal tract indicating that adenoviruses may be part of the normal gut flora. |
format | Online Article Text |
id | pubmed-3184098 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-31840982011-10-06 Adenoviruses in Lymphocytes of the Human Gastro-Intestinal Tract Roy, Soumitra Calcedo, Roberto Medina-Jaszek, Angelica Keough, Martin Peng, Hui Wilson, James M. PLoS One Research Article OBJECTIVE: Persistent adenoviral shedding in stools is known to occur past convalescence following acute adenoviral infections. We wished to establish the frequency with which adenoviruses may colonize the gut in normal human subjects. METHODS: The presence of adenoviral DNA in intestinal specimens obtained at surgery or autopsy was tested using a nested PCR method. The amplified adenoviral DNA sequences were compared to each other and to known adenoviral species. Lamina propria lymphocytes (LPLs) were isolated from the specimens and the adenoviral copy numbers in the CD4+ and CD8+ fractions were determined by quantitative PCR. Adenoviral gene expression was tested by amplification of adenoviral mRNA. RESULTS: Intestinal tissue from 21 of 58 donors and LPLs from 21 of 24 donors were positive for the presence of adenoviral DNA. The majority of the sequences could be assigned to adenoviral species E, although species B and C sequences were also common. Multiple sequences were often present in the same sample. Forty-one non-identical sequences were identified from 39 different tissue donors. Quantitative PCR for adenoviral DNA in CD4+ and CD8+ fractions of LPLs showed adenoviral DNA to be present in both cell types and ranged from a few hundred to several million copies per million cells on average. Active adenoviral gene expression as evidenced by the presence of adenoviral messenger RNA in intestinal lymphocytes was demonstrated in 9 of the 11 donors tested. CONCLUSION: Adenoviral DNA is highly prevalent in lymphocytes from the gastro-intestinal tract indicating that adenoviruses may be part of the normal gut flora. Public Library of Science 2011-09-30 /pmc/articles/PMC3184098/ /pubmed/21980361 http://dx.doi.org/10.1371/journal.pone.0024859 Text en Roy et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Roy, Soumitra Calcedo, Roberto Medina-Jaszek, Angelica Keough, Martin Peng, Hui Wilson, James M. Adenoviruses in Lymphocytes of the Human Gastro-Intestinal Tract |
title | Adenoviruses in Lymphocytes of the Human Gastro-Intestinal Tract |
title_full | Adenoviruses in Lymphocytes of the Human Gastro-Intestinal Tract |
title_fullStr | Adenoviruses in Lymphocytes of the Human Gastro-Intestinal Tract |
title_full_unstemmed | Adenoviruses in Lymphocytes of the Human Gastro-Intestinal Tract |
title_short | Adenoviruses in Lymphocytes of the Human Gastro-Intestinal Tract |
title_sort | adenoviruses in lymphocytes of the human gastro-intestinal tract |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184098/ https://www.ncbi.nlm.nih.gov/pubmed/21980361 http://dx.doi.org/10.1371/journal.pone.0024859 |
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