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Dopamine and Octopamine Influence Avoidance Learning of Honey Bees in a Place Preference Assay

Biogenic amines are widely characterized in pathways evaluating reward and punishment, resulting in appropriate aversive or appetitive responses of vertebrates and invertebrates. We utilized the honey bee model and a newly developed spatial avoidance conditioning assay to probe effects of biogenic a...

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Autores principales: Agarwal, Maitreyi, Giannoni Guzmán, Manuel, Morales-Matos, Carla, Del Valle Díaz, Rafael Alejandro, Abramson, Charles I., Giray, Tugrul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184138/
https://www.ncbi.nlm.nih.gov/pubmed/21980435
http://dx.doi.org/10.1371/journal.pone.0025371
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author Agarwal, Maitreyi
Giannoni Guzmán, Manuel
Morales-Matos, Carla
Del Valle Díaz, Rafael Alejandro
Abramson, Charles I.
Giray, Tugrul
author_facet Agarwal, Maitreyi
Giannoni Guzmán, Manuel
Morales-Matos, Carla
Del Valle Díaz, Rafael Alejandro
Abramson, Charles I.
Giray, Tugrul
author_sort Agarwal, Maitreyi
collection PubMed
description Biogenic amines are widely characterized in pathways evaluating reward and punishment, resulting in appropriate aversive or appetitive responses of vertebrates and invertebrates. We utilized the honey bee model and a newly developed spatial avoidance conditioning assay to probe effects of biogenic amines octopamine (OA) and dopamine (DA) on avoidance learning. In this new protocol non-harnessed bees associate a spatial color cue with mild electric shock punishment. After a number of experiences with color and shock the bees no longer enter the compartment associated with punishment. Intrinsic aspects of avoidance conditioning are associated with natural behavior of bees such as punishment (lack of food, explosive pollination mechanisms, danger of predation, heat, etc.) and their association to floral traits or other spatial cues during foraging. The results show that DA reduces the punishment received whereas octopamine OA increases the punishment received. These effects are dose-dependent and specific to the acquisition phase of training. The effects during acquisition are specific as shown in experiments using the antagonists Pimozide and Mianserin for DA and OA receptors, respectively. This study demonstrates the integrative role of biogenic amines in aversive learning in the honey bee as modeled in a novel non-appetitive avoidance learning assay.
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spelling pubmed-31841382011-10-06 Dopamine and Octopamine Influence Avoidance Learning of Honey Bees in a Place Preference Assay Agarwal, Maitreyi Giannoni Guzmán, Manuel Morales-Matos, Carla Del Valle Díaz, Rafael Alejandro Abramson, Charles I. Giray, Tugrul PLoS One Research Article Biogenic amines are widely characterized in pathways evaluating reward and punishment, resulting in appropriate aversive or appetitive responses of vertebrates and invertebrates. We utilized the honey bee model and a newly developed spatial avoidance conditioning assay to probe effects of biogenic amines octopamine (OA) and dopamine (DA) on avoidance learning. In this new protocol non-harnessed bees associate a spatial color cue with mild electric shock punishment. After a number of experiences with color and shock the bees no longer enter the compartment associated with punishment. Intrinsic aspects of avoidance conditioning are associated with natural behavior of bees such as punishment (lack of food, explosive pollination mechanisms, danger of predation, heat, etc.) and their association to floral traits or other spatial cues during foraging. The results show that DA reduces the punishment received whereas octopamine OA increases the punishment received. These effects are dose-dependent and specific to the acquisition phase of training. The effects during acquisition are specific as shown in experiments using the antagonists Pimozide and Mianserin for DA and OA receptors, respectively. This study demonstrates the integrative role of biogenic amines in aversive learning in the honey bee as modeled in a novel non-appetitive avoidance learning assay. Public Library of Science 2011-09-30 /pmc/articles/PMC3184138/ /pubmed/21980435 http://dx.doi.org/10.1371/journal.pone.0025371 Text en Agarwal et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Agarwal, Maitreyi
Giannoni Guzmán, Manuel
Morales-Matos, Carla
Del Valle Díaz, Rafael Alejandro
Abramson, Charles I.
Giray, Tugrul
Dopamine and Octopamine Influence Avoidance Learning of Honey Bees in a Place Preference Assay
title Dopamine and Octopamine Influence Avoidance Learning of Honey Bees in a Place Preference Assay
title_full Dopamine and Octopamine Influence Avoidance Learning of Honey Bees in a Place Preference Assay
title_fullStr Dopamine and Octopamine Influence Avoidance Learning of Honey Bees in a Place Preference Assay
title_full_unstemmed Dopamine and Octopamine Influence Avoidance Learning of Honey Bees in a Place Preference Assay
title_short Dopamine and Octopamine Influence Avoidance Learning of Honey Bees in a Place Preference Assay
title_sort dopamine and octopamine influence avoidance learning of honey bees in a place preference assay
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184138/
https://www.ncbi.nlm.nih.gov/pubmed/21980435
http://dx.doi.org/10.1371/journal.pone.0025371
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