Cargando…
A Multifunctional 3D Co-Culture System for Studies of Mammary Tissue Morphogenesis and Stem Cell Biology
Studies on the stem cell niche and the efficacy of cancer therapeutics require complex multicellular structures and interactions between different cell types and extracellular matrix (ECM) in three dimensional (3D) space. We have engineered a 3D in vitro model of mammary gland that encompasses a def...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184152/ https://www.ncbi.nlm.nih.gov/pubmed/21984937 http://dx.doi.org/10.1371/journal.pone.0025661 |
_version_ | 1782213070226980864 |
---|---|
author | Campbell, Jonathan J. Davidenko, Natalia Caffarel, Maria M. Cameron, Ruth E. Watson, Christine J. |
author_facet | Campbell, Jonathan J. Davidenko, Natalia Caffarel, Maria M. Cameron, Ruth E. Watson, Christine J. |
author_sort | Campbell, Jonathan J. |
collection | PubMed |
description | Studies on the stem cell niche and the efficacy of cancer therapeutics require complex multicellular structures and interactions between different cell types and extracellular matrix (ECM) in three dimensional (3D) space. We have engineered a 3D in vitro model of mammary gland that encompasses a defined, porous collagen/hyaluronic acid (HA) scaffold forming a physiologically relevant foundation for epithelial and adipocyte co-culture. Polarized ductal and acinar structures form within this scaffold recapitulating normal tissue morphology in the absence of reconstituted basement membrane (rBM) hydrogel. Furthermore, organoid developmental outcome can be controlled by the ratio of collagen to HA, with a higher HA concentration favouring acinar morphological development. Importantly, this culture system recapitulates the stem cell niche as primary mammary stem cells form complex organoids, emphasising the utility of this approach for developmental and tumorigenic studies using genetically altered animals or human biopsy material, and for screening cancer therapeutics for personalised medicine. |
format | Online Article Text |
id | pubmed-3184152 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-31841522011-10-07 A Multifunctional 3D Co-Culture System for Studies of Mammary Tissue Morphogenesis and Stem Cell Biology Campbell, Jonathan J. Davidenko, Natalia Caffarel, Maria M. Cameron, Ruth E. Watson, Christine J. PLoS One Research Article Studies on the stem cell niche and the efficacy of cancer therapeutics require complex multicellular structures and interactions between different cell types and extracellular matrix (ECM) in three dimensional (3D) space. We have engineered a 3D in vitro model of mammary gland that encompasses a defined, porous collagen/hyaluronic acid (HA) scaffold forming a physiologically relevant foundation for epithelial and adipocyte co-culture. Polarized ductal and acinar structures form within this scaffold recapitulating normal tissue morphology in the absence of reconstituted basement membrane (rBM) hydrogel. Furthermore, organoid developmental outcome can be controlled by the ratio of collagen to HA, with a higher HA concentration favouring acinar morphological development. Importantly, this culture system recapitulates the stem cell niche as primary mammary stem cells form complex organoids, emphasising the utility of this approach for developmental and tumorigenic studies using genetically altered animals or human biopsy material, and for screening cancer therapeutics for personalised medicine. Public Library of Science 2011-09-30 /pmc/articles/PMC3184152/ /pubmed/21984937 http://dx.doi.org/10.1371/journal.pone.0025661 Text en Campbell et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Campbell, Jonathan J. Davidenko, Natalia Caffarel, Maria M. Cameron, Ruth E. Watson, Christine J. A Multifunctional 3D Co-Culture System for Studies of Mammary Tissue Morphogenesis and Stem Cell Biology |
title | A Multifunctional 3D Co-Culture System for Studies of Mammary Tissue Morphogenesis and Stem Cell Biology |
title_full | A Multifunctional 3D Co-Culture System for Studies of Mammary Tissue Morphogenesis and Stem Cell Biology |
title_fullStr | A Multifunctional 3D Co-Culture System for Studies of Mammary Tissue Morphogenesis and Stem Cell Biology |
title_full_unstemmed | A Multifunctional 3D Co-Culture System for Studies of Mammary Tissue Morphogenesis and Stem Cell Biology |
title_short | A Multifunctional 3D Co-Culture System for Studies of Mammary Tissue Morphogenesis and Stem Cell Biology |
title_sort | multifunctional 3d co-culture system for studies of mammary tissue morphogenesis and stem cell biology |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184152/ https://www.ncbi.nlm.nih.gov/pubmed/21984937 http://dx.doi.org/10.1371/journal.pone.0025661 |
work_keys_str_mv | AT campbelljonathanj amultifunctional3dcoculturesystemforstudiesofmammarytissuemorphogenesisandstemcellbiology AT davidenkonatalia amultifunctional3dcoculturesystemforstudiesofmammarytissuemorphogenesisandstemcellbiology AT caffarelmariam amultifunctional3dcoculturesystemforstudiesofmammarytissuemorphogenesisandstemcellbiology AT cameronruthe amultifunctional3dcoculturesystemforstudiesofmammarytissuemorphogenesisandstemcellbiology AT watsonchristinej amultifunctional3dcoculturesystemforstudiesofmammarytissuemorphogenesisandstemcellbiology AT campbelljonathanj multifunctional3dcoculturesystemforstudiesofmammarytissuemorphogenesisandstemcellbiology AT davidenkonatalia multifunctional3dcoculturesystemforstudiesofmammarytissuemorphogenesisandstemcellbiology AT caffarelmariam multifunctional3dcoculturesystemforstudiesofmammarytissuemorphogenesisandstemcellbiology AT cameronruthe multifunctional3dcoculturesystemforstudiesofmammarytissuemorphogenesisandstemcellbiology AT watsonchristinej multifunctional3dcoculturesystemforstudiesofmammarytissuemorphogenesisandstemcellbiology |