Simple and Specific Dual-Wavelength Excitable Dye Staining for Glycoprotein Detection in Polyacrylamide Gels and Its Application in Glycoproteomics

In this study, a commercially available fluorescent dye, Lissamine rhodamine B sulfonyl hydrazine (LRSH), was designed to specifically stain the glycoproteins in polyacrylamide gels. Through the periodate/Schiff base mechanism, the fluorescent dye readily attaches to glycoproteins and the fluorescen...

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Detalles Bibliográficos
Autores principales: Chiang, Yu-Hsuan, Wu, Yu-Jen, Lu, Ya-Ting, Chen, Kuan-Hung, Lin, Tzu-Chun, Chen, Yu-Kuang H., Li, Ding-Tzai, Shi, Fong-Ku, Chen, Ching-Chuan, Hsu, Jue-Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Methodology Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184442/
https://www.ncbi.nlm.nih.gov/pubmed/21976968
http://dx.doi.org/10.1155/2011/780108

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