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Effects of incomplete decay in fluorescence lifetime estimation

Fluorescence lifetime imaging has emerged as an important microscopy technique, where high repetition rate lasers are the primary light sources. As fluorescence lifetime becomes comparable to intervals between consecutive excitation pulses, incomplete fluorescence decay from previous pulses can supe...

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Detalles Bibliográficos
Autores principales: Leung, Regina Won Kay, Yeh, Shu-Chi Allison, Fang, Qiyin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184861/
https://www.ncbi.nlm.nih.gov/pubmed/21991544
http://dx.doi.org/10.1364/BOE.2.002517
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author Leung, Regina Won Kay
Yeh, Shu-Chi Allison
Fang, Qiyin
author_facet Leung, Regina Won Kay
Yeh, Shu-Chi Allison
Fang, Qiyin
author_sort Leung, Regina Won Kay
collection PubMed
description Fluorescence lifetime imaging has emerged as an important microscopy technique, where high repetition rate lasers are the primary light sources. As fluorescence lifetime becomes comparable to intervals between consecutive excitation pulses, incomplete fluorescence decay from previous pulses can superimpose onto the subsequent decay measurements. Using a mathematical model, the incomplete decay effect has been shown to lead to overestimation of the amplitude average lifetime except in mono-exponential decays. An inverse model is then developed to correct the error from this effect and the theoretical simulations are tested by experimental results.
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spelling pubmed-31848612011-10-11 Effects of incomplete decay in fluorescence lifetime estimation Leung, Regina Won Kay Yeh, Shu-Chi Allison Fang, Qiyin Biomed Opt Express Spectroscopic Diagnostics Fluorescence lifetime imaging has emerged as an important microscopy technique, where high repetition rate lasers are the primary light sources. As fluorescence lifetime becomes comparable to intervals between consecutive excitation pulses, incomplete fluorescence decay from previous pulses can superimpose onto the subsequent decay measurements. Using a mathematical model, the incomplete decay effect has been shown to lead to overestimation of the amplitude average lifetime except in mono-exponential decays. An inverse model is then developed to correct the error from this effect and the theoretical simulations are tested by experimental results. Optical Society of America 2011-08-02 /pmc/articles/PMC3184861/ /pubmed/21991544 http://dx.doi.org/10.1364/BOE.2.002517 Text en ©2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Spectroscopic Diagnostics
Leung, Regina Won Kay
Yeh, Shu-Chi Allison
Fang, Qiyin
Effects of incomplete decay in fluorescence lifetime estimation
title Effects of incomplete decay in fluorescence lifetime estimation
title_full Effects of incomplete decay in fluorescence lifetime estimation
title_fullStr Effects of incomplete decay in fluorescence lifetime estimation
title_full_unstemmed Effects of incomplete decay in fluorescence lifetime estimation
title_short Effects of incomplete decay in fluorescence lifetime estimation
title_sort effects of incomplete decay in fluorescence lifetime estimation
topic Spectroscopic Diagnostics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184861/
https://www.ncbi.nlm.nih.gov/pubmed/21991544
http://dx.doi.org/10.1364/BOE.2.002517
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