Holographic UV laser microsurgery

ABSTRACT: We use a spatial light modulator (SLM) to diffract a single UV laser pulse to ablate multiple points on a Drosophila embryo. This system dynamically generates a phase hologram for ablating a user-defined pattern fast enough to be used with living, and thus moving, tissue. We demonstrate th...

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Detalles Bibliográficos
Autores principales: Jayasinghe, Aroshan K, Rohner, Jason, Hutson, M Shane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2011
Materias:
Optical Therapies and Photomodificaton
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184868/
https://www.ncbi.nlm.nih.gov/pubmed/21991551
http://dx.doi.org/10.1364/BOE.2.002590
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author Jayasinghe, Aroshan K
Rohner, Jason
Hutson, M Shane
author_facet Jayasinghe, Aroshan K
Rohner, Jason
Hutson, M Shane
author_sort Jayasinghe, Aroshan K
collection PubMed
description ABSTRACT: We use a spatial light modulator (SLM) to diffract a single UV laser pulse to ablate multiple points on a Drosophila embryo. This system dynamically generates a phase hologram for ablating a user-defined pattern fast enough to be used with living, and thus moving, tissue. We demonstrate the ability of this single-pulse multi-point system to perform two experiments that are very difficult for conventional microsurgery—isolating single cells in vivo and measuring fast retractions from large incisions.
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spelling pubmed-31848682011-10-11 Holographic UV laser microsurgery Jayasinghe, Aroshan K Rohner, Jason Hutson, M Shane Biomed Opt Express Optical Therapies and Photomodificaton ABSTRACT: We use a spatial light modulator (SLM) to diffract a single UV laser pulse to ablate multiple points on a Drosophila embryo. This system dynamically generates a phase hologram for ablating a user-defined pattern fast enough to be used with living, and thus moving, tissue. We demonstrate the ability of this single-pulse multi-point system to perform two experiments that are very difficult for conventional microsurgery—isolating single cells in vivo and measuring fast retractions from large incisions. Optical Society of America 2011-08-15 /pmc/articles/PMC3184868/ /pubmed/21991551 http://dx.doi.org/10.1364/BOE.2.002590 Text en ©2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Optical Therapies and Photomodificaton
Jayasinghe, Aroshan K
Rohner, Jason
Hutson, M Shane
Holographic UV laser microsurgery
title Holographic UV laser microsurgery
title_full Holographic UV laser microsurgery
title_fullStr Holographic UV laser microsurgery
title_full_unstemmed Holographic UV laser microsurgery
title_short Holographic UV laser microsurgery
title_sort holographic uv laser microsurgery
topic Optical Therapies and Photomodificaton
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184868/
https://www.ncbi.nlm.nih.gov/pubmed/21991551
http://dx.doi.org/10.1364/BOE.2.002590
work_keys_str_mv AT jayasinghearoshank holographicuvlasermicrosurgery
AT rohnerjason holographicuvlasermicrosurgery
AT hutsonmshane holographicuvlasermicrosurgery

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