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In vivo evaluation of demyelination and remyelination in a nerve crush injury model
Nerves of the peripheral nervous system have, to some extent, the ability to regenerate after injury, particularly in instances of crush or contusion injuries. After a controlled crush injury of the rat sciatic nerve, demyelination and remyelination are followed with functional assessments and image...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Optical Society of America
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184878/ https://www.ncbi.nlm.nih.gov/pubmed/22091449 http://dx.doi.org/10.1364/BOE.2.002698 |
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author | Bélanger, E. Henry, F. P. Vallée, R. Randolph, M. A. Kochevar, I. E. Winograd, J. M. Lin, C. P. Côté, D. |
author_facet | Bélanger, E. Henry, F. P. Vallée, R. Randolph, M. A. Kochevar, I. E. Winograd, J. M. Lin, C. P. Côté, D. |
author_sort | Bélanger, E. |
collection | PubMed |
description | Nerves of the peripheral nervous system have, to some extent, the ability to regenerate after injury, particularly in instances of crush or contusion injuries. After a controlled crush injury of the rat sciatic nerve, demyelination and remyelination are followed with functional assessments and imaged both ex vivo and in vivo over the course of 4 weeks with video-rate coherent anti-Stokes Raman scattering (CARS) microscopy. A new procedure compatible with live animal imaging is developed for performing histomorphometry of myelinated axons. This allows quantification of demyelination proximal and remyelination distal to the crush site ex vivo and in vivo respectively. |
format | Online Article Text |
id | pubmed-3184878 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Optical Society of America |
record_format | MEDLINE/PubMed |
spelling | pubmed-31848782011-11-16 In vivo evaluation of demyelination and remyelination in a nerve crush injury model Bélanger, E. Henry, F. P. Vallée, R. Randolph, M. A. Kochevar, I. E. Winograd, J. M. Lin, C. P. Côté, D. Biomed Opt Express Neuroscience and Brain Imaging Nerves of the peripheral nervous system have, to some extent, the ability to regenerate after injury, particularly in instances of crush or contusion injuries. After a controlled crush injury of the rat sciatic nerve, demyelination and remyelination are followed with functional assessments and imaged both ex vivo and in vivo over the course of 4 weeks with video-rate coherent anti-Stokes Raman scattering (CARS) microscopy. A new procedure compatible with live animal imaging is developed for performing histomorphometry of myelinated axons. This allows quantification of demyelination proximal and remyelination distal to the crush site ex vivo and in vivo respectively. Optical Society of America 2011-08-24 /pmc/articles/PMC3184878/ /pubmed/22091449 http://dx.doi.org/10.1364/BOE.2.002698 Text en © 2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially. |
spellingShingle | Neuroscience and Brain Imaging Bélanger, E. Henry, F. P. Vallée, R. Randolph, M. A. Kochevar, I. E. Winograd, J. M. Lin, C. P. Côté, D. In vivo evaluation of demyelination and remyelination in a nerve crush injury model |
title | In vivo evaluation of demyelination and remyelination in a nerve crush injury model |
title_full | In vivo evaluation of demyelination and remyelination in a nerve crush injury model |
title_fullStr | In vivo evaluation of demyelination and remyelination in a nerve crush injury model |
title_full_unstemmed | In vivo evaluation of demyelination and remyelination in a nerve crush injury model |
title_short | In vivo evaluation of demyelination and remyelination in a nerve crush injury model |
title_sort | in vivo evaluation of demyelination and remyelination in a nerve crush injury model |
topic | Neuroscience and Brain Imaging |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184878/ https://www.ncbi.nlm.nih.gov/pubmed/22091449 http://dx.doi.org/10.1364/BOE.2.002698 |
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