Cargando…

Development of a highly specific amine-terminated aptamer functionalized surface plasmon resonance biosensor for blood protein detection

This paper presents a generally applicable approach for the highly specific detection of blood proteins. Thrombin and thrombin-binding aptamers are chosen for demonstration purposes. The sensor was prepared by immobilizing amine-terminated aptamers onto a gold modified surface using a two-step self-...

Descripción completa

Detalles Bibliográficos
Autores principales: Zheng, Rui, Park, Byung-Wook, Kim, Dong-Shik, Cameron, Brent D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184881/
https://www.ncbi.nlm.nih.gov/pubmed/21991560
http://dx.doi.org/10.1364/BOE.2.002731
_version_ 1782213159552024576
author Zheng, Rui
Park, Byung-Wook
Kim, Dong-Shik
Cameron, Brent D.
author_facet Zheng, Rui
Park, Byung-Wook
Kim, Dong-Shik
Cameron, Brent D.
author_sort Zheng, Rui
collection PubMed
description This paper presents a generally applicable approach for the highly specific detection of blood proteins. Thrombin and thrombin-binding aptamers are chosen for demonstration purposes. The sensor was prepared by immobilizing amine-terminated aptamers onto a gold modified surface using a two-step self-assembled monolayer (SAM) immobilization technique and the physical detection is performed using Surface Plasmon Resonance (SPR). The developed sensor has an optimal detectable range of 5–1000 nM and the results show the sensor has good reversibility, sensitivity and selectivity. Furthermore, the sensor shows the potential of being improved and standardized for direct detection of other blood proteins for clinical applications.
format Online
Article
Text
id pubmed-3184881
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Optical Society of America
record_format MEDLINE/PubMed
spelling pubmed-31848812011-10-11 Development of a highly specific amine-terminated aptamer functionalized surface plasmon resonance biosensor for blood protein detection Zheng, Rui Park, Byung-Wook Kim, Dong-Shik Cameron, Brent D. Biomed Opt Express Biosensors and Molecular Diagnostics This paper presents a generally applicable approach for the highly specific detection of blood proteins. Thrombin and thrombin-binding aptamers are chosen for demonstration purposes. The sensor was prepared by immobilizing amine-terminated aptamers onto a gold modified surface using a two-step self-assembled monolayer (SAM) immobilization technique and the physical detection is performed using Surface Plasmon Resonance (SPR). The developed sensor has an optimal detectable range of 5–1000 nM and the results show the sensor has good reversibility, sensitivity and selectivity. Furthermore, the sensor shows the potential of being improved and standardized for direct detection of other blood proteins for clinical applications. Optical Society of America 2011-08-31 /pmc/articles/PMC3184881/ /pubmed/21991560 http://dx.doi.org/10.1364/BOE.2.002731 Text en ©2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Biosensors and Molecular Diagnostics
Zheng, Rui
Park, Byung-Wook
Kim, Dong-Shik
Cameron, Brent D.
Development of a highly specific amine-terminated aptamer functionalized surface plasmon resonance biosensor for blood protein detection
title Development of a highly specific amine-terminated aptamer functionalized surface plasmon resonance biosensor for blood protein detection
title_full Development of a highly specific amine-terminated aptamer functionalized surface plasmon resonance biosensor for blood protein detection
title_fullStr Development of a highly specific amine-terminated aptamer functionalized surface plasmon resonance biosensor for blood protein detection
title_full_unstemmed Development of a highly specific amine-terminated aptamer functionalized surface plasmon resonance biosensor for blood protein detection
title_short Development of a highly specific amine-terminated aptamer functionalized surface plasmon resonance biosensor for blood protein detection
title_sort development of a highly specific amine-terminated aptamer functionalized surface plasmon resonance biosensor for blood protein detection
topic Biosensors and Molecular Diagnostics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3184881/
https://www.ncbi.nlm.nih.gov/pubmed/21991560
http://dx.doi.org/10.1364/BOE.2.002731
work_keys_str_mv AT zhengrui developmentofahighlyspecificamineterminatedaptamerfunctionalizedsurfaceplasmonresonancebiosensorforbloodproteindetection
AT parkbyungwook developmentofahighlyspecificamineterminatedaptamerfunctionalizedsurfaceplasmonresonancebiosensorforbloodproteindetection
AT kimdongshik developmentofahighlyspecificamineterminatedaptamerfunctionalizedsurfaceplasmonresonancebiosensorforbloodproteindetection
AT cameronbrentd developmentofahighlyspecificamineterminatedaptamerfunctionalizedsurfaceplasmonresonancebiosensorforbloodproteindetection