Cargando…

The chicken cornea as a model of wound healing and neuronal re-innervation

PURPOSE: The cornea is the major refractive component of the eye and serves as a barrier to the external environment. Understanding how the cornea responds to injury is important to developing therapies to treat vision disorders that affect the integrity and refractive properties of the cornea. Thus...

Descripción completa

Detalles Bibliográficos
Autores principales: Ritchey, Eric R., Code, Kimberly, Zelinka, Christopher P., Scott, Melissa A., Fischer, Andy J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Vision 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3185018/
https://www.ncbi.nlm.nih.gov/pubmed/21976955
_version_ 1782213179530543104
author Ritchey, Eric R.
Code, Kimberly
Zelinka, Christopher P.
Scott, Melissa A.
Fischer, Andy J.
author_facet Ritchey, Eric R.
Code, Kimberly
Zelinka, Christopher P.
Scott, Melissa A.
Fischer, Andy J.
author_sort Ritchey, Eric R.
collection PubMed
description PURPOSE: The cornea is the major refractive component of the eye and serves as a barrier to the external environment. Understanding how the cornea responds to injury is important to developing therapies to treat vision disorders that affect the integrity and refractive properties of the cornea. Thus, investigation of the wound healing responses of the cornea to injury in a cost-effective animal model is a valuable tool for research. This study characterizes the wound healing responses in the corneas of White Leghorn chicken. METHODS: Linear corneal wounds were induced in post-natal day 7 (P7) chicks and cellular proliferation, apoptosis and regulation of structural proteins were assessed using immunohistochemical techniques. We describe the time course of increased expression of different scar-related markers, including vimentin, vinculin, perlecan and smooth muscle actin. RESULTS: We find evidence for acute necrotic cell death in the corneal region immediately surrounding cite of incision, whereas we failed to find evidence of delayed cell death or apoptosis. We find that the neuronal re-innervation of SV2-positive axon terminals within the corneal stroma and epithelium occurs very quickly after the initial scarring insult. We describe an accumulation of cells within the stroma immediately underlying the scar, which results, at least in part, from the local proliferation of keratocytes. Further, we provide evidence for scar-induced accumulations of CD45-positive monocytes in injured corneas. CONCLUSIONS: We conclude that the chick cornea is an excellent model system in which to study wound healing, formation of scar tissue, and neuronal re-innervation of sensory endings.
format Online
Article
Text
id pubmed-3185018
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Molecular Vision
record_format MEDLINE/PubMed
spelling pubmed-31850182011-10-04 The chicken cornea as a model of wound healing and neuronal re-innervation Ritchey, Eric R. Code, Kimberly Zelinka, Christopher P. Scott, Melissa A. Fischer, Andy J. Mol Vis Research Article PURPOSE: The cornea is the major refractive component of the eye and serves as a barrier to the external environment. Understanding how the cornea responds to injury is important to developing therapies to treat vision disorders that affect the integrity and refractive properties of the cornea. Thus, investigation of the wound healing responses of the cornea to injury in a cost-effective animal model is a valuable tool for research. This study characterizes the wound healing responses in the corneas of White Leghorn chicken. METHODS: Linear corneal wounds were induced in post-natal day 7 (P7) chicks and cellular proliferation, apoptosis and regulation of structural proteins were assessed using immunohistochemical techniques. We describe the time course of increased expression of different scar-related markers, including vimentin, vinculin, perlecan and smooth muscle actin. RESULTS: We find evidence for acute necrotic cell death in the corneal region immediately surrounding cite of incision, whereas we failed to find evidence of delayed cell death or apoptosis. We find that the neuronal re-innervation of SV2-positive axon terminals within the corneal stroma and epithelium occurs very quickly after the initial scarring insult. We describe an accumulation of cells within the stroma immediately underlying the scar, which results, at least in part, from the local proliferation of keratocytes. Further, we provide evidence for scar-induced accumulations of CD45-positive monocytes in injured corneas. CONCLUSIONS: We conclude that the chick cornea is an excellent model system in which to study wound healing, formation of scar tissue, and neuronal re-innervation of sensory endings. Molecular Vision 2011-09-21 /pmc/articles/PMC3185018/ /pubmed/21976955 Text en Copyright © 2011 Molecular Vision. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ritchey, Eric R.
Code, Kimberly
Zelinka, Christopher P.
Scott, Melissa A.
Fischer, Andy J.
The chicken cornea as a model of wound healing and neuronal re-innervation
title The chicken cornea as a model of wound healing and neuronal re-innervation
title_full The chicken cornea as a model of wound healing and neuronal re-innervation
title_fullStr The chicken cornea as a model of wound healing and neuronal re-innervation
title_full_unstemmed The chicken cornea as a model of wound healing and neuronal re-innervation
title_short The chicken cornea as a model of wound healing and neuronal re-innervation
title_sort chicken cornea as a model of wound healing and neuronal re-innervation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3185018/
https://www.ncbi.nlm.nih.gov/pubmed/21976955
work_keys_str_mv AT ritcheyericr thechickencorneaasamodelofwoundhealingandneuronalreinnervation
AT codekimberly thechickencorneaasamodelofwoundhealingandneuronalreinnervation
AT zelinkachristopherp thechickencorneaasamodelofwoundhealingandneuronalreinnervation
AT scottmelissaa thechickencorneaasamodelofwoundhealingandneuronalreinnervation
AT fischerandyj thechickencorneaasamodelofwoundhealingandneuronalreinnervation
AT ritcheyericr chickencorneaasamodelofwoundhealingandneuronalreinnervation
AT codekimberly chickencorneaasamodelofwoundhealingandneuronalreinnervation
AT zelinkachristopherp chickencorneaasamodelofwoundhealingandneuronalreinnervation
AT scottmelissaa chickencorneaasamodelofwoundhealingandneuronalreinnervation
AT fischerandyj chickencorneaasamodelofwoundhealingandneuronalreinnervation