Complete Nucleotide Sequence and Molecular Characterization of Bacillus Phage TP21 and its Relatedness to Other Phages with the Same Name

Three different Bacillus bacteriophages designated TP21 are known from the literature. We have determined the sequence and structure of the TP21-L genome, and compared it to the other phages. The genome is 37.5 kb in size, possesses fixed invariable genome ends and features the typical modular organ...

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Autores principales: Klumpp, Jochen, Calendar, Richard, Loessner, Martin J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3185655/
https://www.ncbi.nlm.nih.gov/pubmed/21994663
http://dx.doi.org/10.3390/v2040961
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author Klumpp, Jochen
Calendar, Richard
Loessner, Martin J.
author_facet Klumpp, Jochen
Calendar, Richard
Loessner, Martin J.
author_sort Klumpp, Jochen
collection PubMed
description Three different Bacillus bacteriophages designated TP21 are known from the literature. We have determined the sequence and structure of the TP21-L genome, and compared it to the other phages. The genome is 37.5 kb in size, possesses fixed invariable genome ends and features the typical modular organization of a temperate siphovirus. TP21-L is neither identical to TP21 isolated by Thorne (TP21-T), as shown by a PCR-based approach nor to TP21 isolated by He et al. (TP21-H), as estimated from phage dimensions. For reasons of clarity, we suggest renaming the different TP21 isolates.
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spelling pubmed-31856552011-10-12 Complete Nucleotide Sequence and Molecular Characterization of Bacillus Phage TP21 and its Relatedness to Other Phages with the Same Name Klumpp, Jochen Calendar, Richard Loessner, Martin J. Viruses Article Three different Bacillus bacteriophages designated TP21 are known from the literature. We have determined the sequence and structure of the TP21-L genome, and compared it to the other phages. The genome is 37.5 kb in size, possesses fixed invariable genome ends and features the typical modular organization of a temperate siphovirus. TP21-L is neither identical to TP21 isolated by Thorne (TP21-T), as shown by a PCR-based approach nor to TP21 isolated by He et al. (TP21-H), as estimated from phage dimensions. For reasons of clarity, we suggest renaming the different TP21 isolates. Molecular Diversity Preservation International (MDPI) 2010-04-06 /pmc/articles/PMC3185655/ /pubmed/21994663 http://dx.doi.org/10.3390/v2040961 Text en © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Klumpp, Jochen
Calendar, Richard
Loessner, Martin J.
Complete Nucleotide Sequence and Molecular Characterization of Bacillus Phage TP21 and its Relatedness to Other Phages with the Same Name
title Complete Nucleotide Sequence and Molecular Characterization of Bacillus Phage TP21 and its Relatedness to Other Phages with the Same Name
title_full Complete Nucleotide Sequence and Molecular Characterization of Bacillus Phage TP21 and its Relatedness to Other Phages with the Same Name
title_fullStr Complete Nucleotide Sequence and Molecular Characterization of Bacillus Phage TP21 and its Relatedness to Other Phages with the Same Name
title_full_unstemmed Complete Nucleotide Sequence and Molecular Characterization of Bacillus Phage TP21 and its Relatedness to Other Phages with the Same Name
title_short Complete Nucleotide Sequence and Molecular Characterization of Bacillus Phage TP21 and its Relatedness to Other Phages with the Same Name
title_sort complete nucleotide sequence and molecular characterization of bacillus phage tp21 and its relatedness to other phages with the same name
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3185655/
https://www.ncbi.nlm.nih.gov/pubmed/21994663
http://dx.doi.org/10.3390/v2040961
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