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Gene Therapy with Helper-Dependent Adenoviral Vectors: Current Advances and Future Perspectives

Recombinant Adenoviral vectors represent one of the best gene transfer platforms due to their ability to efficiently transduce a wide range of quiescent and proliferating cell types from various tissues and species. The activation of an adaptive immune response against the transduced cells is one of...

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Detalles Bibliográficos
Autores principales: Vetrini, Francesco, Ng, Philip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3186006/
https://www.ncbi.nlm.nih.gov/pubmed/21994713
http://dx.doi.org/10.3390/v2091886
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author Vetrini, Francesco
Ng, Philip
author_facet Vetrini, Francesco
Ng, Philip
author_sort Vetrini, Francesco
collection PubMed
description Recombinant Adenoviral vectors represent one of the best gene transfer platforms due to their ability to efficiently transduce a wide range of quiescent and proliferating cell types from various tissues and species. The activation of an adaptive immune response against the transduced cells is one of the major drawbacks of first generation Adenovirus vectors and has been overcome by the latest generation of recombinant Adenovirus, the Helper-Dependent Adenoviral (HDAd) vectors. HDAds have innovative features including the complete absence of viral coding sequences and the ability to mediate high level transgene expression with negligible chronic toxicity. This review summarizes the many aspects of HDAd biology and structure with a major focus on in vivo gene therapy application and with an emphasis on the unsolved issues that these vectors still presents toward clinical application.
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spelling pubmed-31860062011-10-12 Gene Therapy with Helper-Dependent Adenoviral Vectors: Current Advances and Future Perspectives Vetrini, Francesco Ng, Philip Viruses Review Recombinant Adenoviral vectors represent one of the best gene transfer platforms due to their ability to efficiently transduce a wide range of quiescent and proliferating cell types from various tissues and species. The activation of an adaptive immune response against the transduced cells is one of the major drawbacks of first generation Adenovirus vectors and has been overcome by the latest generation of recombinant Adenovirus, the Helper-Dependent Adenoviral (HDAd) vectors. HDAds have innovative features including the complete absence of viral coding sequences and the ability to mediate high level transgene expression with negligible chronic toxicity. This review summarizes the many aspects of HDAd biology and structure with a major focus on in vivo gene therapy application and with an emphasis on the unsolved issues that these vectors still presents toward clinical application. Molecular Diversity Preservation International (MDPI) 2010-09-03 /pmc/articles/PMC3186006/ /pubmed/21994713 http://dx.doi.org/10.3390/v2091886 Text en © 2010 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Vetrini, Francesco
Ng, Philip
Gene Therapy with Helper-Dependent Adenoviral Vectors: Current Advances and Future Perspectives
title Gene Therapy with Helper-Dependent Adenoviral Vectors: Current Advances and Future Perspectives
title_full Gene Therapy with Helper-Dependent Adenoviral Vectors: Current Advances and Future Perspectives
title_fullStr Gene Therapy with Helper-Dependent Adenoviral Vectors: Current Advances and Future Perspectives
title_full_unstemmed Gene Therapy with Helper-Dependent Adenoviral Vectors: Current Advances and Future Perspectives
title_short Gene Therapy with Helper-Dependent Adenoviral Vectors: Current Advances and Future Perspectives
title_sort gene therapy with helper-dependent adenoviral vectors: current advances and future perspectives
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3186006/
https://www.ncbi.nlm.nih.gov/pubmed/21994713
http://dx.doi.org/10.3390/v2091886
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