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Actin and microtubules drive differential aspects of planar cell polarity in multiciliated cells
Planar cell polarization represents the ability of cells to orient within the plane of a tissue orthogonal to the apical basal axis. The proper polarized function of multiciliated cells requires the coordination of cilia spacing and cilia polarity as well as the timing of cilia beating during metach...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3187709/ https://www.ncbi.nlm.nih.gov/pubmed/21949415 http://dx.doi.org/10.1083/jcb.201106110 |
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author | Werner, Michael E. Hwang, Peter Huisman, Fawn Taborek, Peter Yu, Clare C. Mitchell, Brian J. |
author_facet | Werner, Michael E. Hwang, Peter Huisman, Fawn Taborek, Peter Yu, Clare C. Mitchell, Brian J. |
author_sort | Werner, Michael E. |
collection | PubMed |
description | Planar cell polarization represents the ability of cells to orient within the plane of a tissue orthogonal to the apical basal axis. The proper polarized function of multiciliated cells requires the coordination of cilia spacing and cilia polarity as well as the timing of cilia beating during metachronal synchrony. The planar cell polarity pathway and hydrodynamic forces have been shown to instruct cilia polarity. In this paper, we show how intracellular effectors interpret polarity to organize cellular morphology in accordance with asymmetric cellular function. We observe that both cellular actin and microtubule networks undergo drastic reorganization, providing differential roles during the polarized organization of cilia. Using computational angular correlation analysis of cilia orientation, we report a graded cellular organization downstream of cell polarity cues. Actin dynamics are required for proper cilia spacing, global coordination of cilia polarity, and coordination of metachronic cilia beating, whereas cytoplasmic microtubule dynamics are required for local coordination of polarity between neighboring cilia. |
format | Online Article Text |
id | pubmed-3187709 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-31877092012-04-03 Actin and microtubules drive differential aspects of planar cell polarity in multiciliated cells Werner, Michael E. Hwang, Peter Huisman, Fawn Taborek, Peter Yu, Clare C. Mitchell, Brian J. J Cell Biol Research Articles Planar cell polarization represents the ability of cells to orient within the plane of a tissue orthogonal to the apical basal axis. The proper polarized function of multiciliated cells requires the coordination of cilia spacing and cilia polarity as well as the timing of cilia beating during metachronal synchrony. The planar cell polarity pathway and hydrodynamic forces have been shown to instruct cilia polarity. In this paper, we show how intracellular effectors interpret polarity to organize cellular morphology in accordance with asymmetric cellular function. We observe that both cellular actin and microtubule networks undergo drastic reorganization, providing differential roles during the polarized organization of cilia. Using computational angular correlation analysis of cilia orientation, we report a graded cellular organization downstream of cell polarity cues. Actin dynamics are required for proper cilia spacing, global coordination of cilia polarity, and coordination of metachronic cilia beating, whereas cytoplasmic microtubule dynamics are required for local coordination of polarity between neighboring cilia. The Rockefeller University Press 2011-10-03 /pmc/articles/PMC3187709/ /pubmed/21949415 http://dx.doi.org/10.1083/jcb.201106110 Text en © 2011 Werner et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Werner, Michael E. Hwang, Peter Huisman, Fawn Taborek, Peter Yu, Clare C. Mitchell, Brian J. Actin and microtubules drive differential aspects of planar cell polarity in multiciliated cells |
title | Actin and microtubules drive differential aspects of planar cell polarity in multiciliated cells |
title_full | Actin and microtubules drive differential aspects of planar cell polarity in multiciliated cells |
title_fullStr | Actin and microtubules drive differential aspects of planar cell polarity in multiciliated cells |
title_full_unstemmed | Actin and microtubules drive differential aspects of planar cell polarity in multiciliated cells |
title_short | Actin and microtubules drive differential aspects of planar cell polarity in multiciliated cells |
title_sort | actin and microtubules drive differential aspects of planar cell polarity in multiciliated cells |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3187709/ https://www.ncbi.nlm.nih.gov/pubmed/21949415 http://dx.doi.org/10.1083/jcb.201106110 |
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