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Actin and microtubules drive differential aspects of planar cell polarity in multiciliated cells

Planar cell polarization represents the ability of cells to orient within the plane of a tissue orthogonal to the apical basal axis. The proper polarized function of multiciliated cells requires the coordination of cilia spacing and cilia polarity as well as the timing of cilia beating during metach...

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Autores principales: Werner, Michael E., Hwang, Peter, Huisman, Fawn, Taborek, Peter, Yu, Clare C., Mitchell, Brian J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3187709/
https://www.ncbi.nlm.nih.gov/pubmed/21949415
http://dx.doi.org/10.1083/jcb.201106110
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author Werner, Michael E.
Hwang, Peter
Huisman, Fawn
Taborek, Peter
Yu, Clare C.
Mitchell, Brian J.
author_facet Werner, Michael E.
Hwang, Peter
Huisman, Fawn
Taborek, Peter
Yu, Clare C.
Mitchell, Brian J.
author_sort Werner, Michael E.
collection PubMed
description Planar cell polarization represents the ability of cells to orient within the plane of a tissue orthogonal to the apical basal axis. The proper polarized function of multiciliated cells requires the coordination of cilia spacing and cilia polarity as well as the timing of cilia beating during metachronal synchrony. The planar cell polarity pathway and hydrodynamic forces have been shown to instruct cilia polarity. In this paper, we show how intracellular effectors interpret polarity to organize cellular morphology in accordance with asymmetric cellular function. We observe that both cellular actin and microtubule networks undergo drastic reorganization, providing differential roles during the polarized organization of cilia. Using computational angular correlation analysis of cilia orientation, we report a graded cellular organization downstream of cell polarity cues. Actin dynamics are required for proper cilia spacing, global coordination of cilia polarity, and coordination of metachronic cilia beating, whereas cytoplasmic microtubule dynamics are required for local coordination of polarity between neighboring cilia.
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spelling pubmed-31877092012-04-03 Actin and microtubules drive differential aspects of planar cell polarity in multiciliated cells Werner, Michael E. Hwang, Peter Huisman, Fawn Taborek, Peter Yu, Clare C. Mitchell, Brian J. J Cell Biol Research Articles Planar cell polarization represents the ability of cells to orient within the plane of a tissue orthogonal to the apical basal axis. The proper polarized function of multiciliated cells requires the coordination of cilia spacing and cilia polarity as well as the timing of cilia beating during metachronal synchrony. The planar cell polarity pathway and hydrodynamic forces have been shown to instruct cilia polarity. In this paper, we show how intracellular effectors interpret polarity to organize cellular morphology in accordance with asymmetric cellular function. We observe that both cellular actin and microtubule networks undergo drastic reorganization, providing differential roles during the polarized organization of cilia. Using computational angular correlation analysis of cilia orientation, we report a graded cellular organization downstream of cell polarity cues. Actin dynamics are required for proper cilia spacing, global coordination of cilia polarity, and coordination of metachronic cilia beating, whereas cytoplasmic microtubule dynamics are required for local coordination of polarity between neighboring cilia. The Rockefeller University Press 2011-10-03 /pmc/articles/PMC3187709/ /pubmed/21949415 http://dx.doi.org/10.1083/jcb.201106110 Text en © 2011 Werner et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Werner, Michael E.
Hwang, Peter
Huisman, Fawn
Taborek, Peter
Yu, Clare C.
Mitchell, Brian J.
Actin and microtubules drive differential aspects of planar cell polarity in multiciliated cells
title Actin and microtubules drive differential aspects of planar cell polarity in multiciliated cells
title_full Actin and microtubules drive differential aspects of planar cell polarity in multiciliated cells
title_fullStr Actin and microtubules drive differential aspects of planar cell polarity in multiciliated cells
title_full_unstemmed Actin and microtubules drive differential aspects of planar cell polarity in multiciliated cells
title_short Actin and microtubules drive differential aspects of planar cell polarity in multiciliated cells
title_sort actin and microtubules drive differential aspects of planar cell polarity in multiciliated cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3187709/
https://www.ncbi.nlm.nih.gov/pubmed/21949415
http://dx.doi.org/10.1083/jcb.201106110
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