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Genetic Variations in Plasma Circulating DNA of HBV-Related Hepatocellular Carcinoma Patients Predict Recurrence after Liver Transplantation

BACKGROUND: Recurrence prediction of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) patients undergoing liver transplantation (LT) present a great challenge because of a lack of biomarkers. Genetic variations play an important role in tumor development and metastasis. METHODS: Oligon...

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Autores principales: Hu, Jie, Wang, Zheng, Fan, Jia, Dai, Zhi, He, Yi-Feng, Qiu, Shuang-Jian, Huang, Xiao-Wu, Sun, Jian, Xiao, Yong-Sheng, Song, Kang, Shi, Ying-Hong, Sun, Qi-Man, Yang, Xin-Rong, Shi, Guo-Ming, Yu, Lei, Yang, Guo-Huan, Ding, Zhen-Bin, Gao, Qiang, Tang, Zhao-You, Zhou, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3187841/
https://www.ncbi.nlm.nih.gov/pubmed/21998744
http://dx.doi.org/10.1371/journal.pone.0026003
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author Hu, Jie
Wang, Zheng
Fan, Jia
Dai, Zhi
He, Yi-Feng
Qiu, Shuang-Jian
Huang, Xiao-Wu
Sun, Jian
Xiao, Yong-Sheng
Song, Kang
Shi, Ying-Hong
Sun, Qi-Man
Yang, Xin-Rong
Shi, Guo-Ming
Yu, Lei
Yang, Guo-Huan
Ding, Zhen-Bin
Gao, Qiang
Tang, Zhao-You
Zhou, Jian
author_facet Hu, Jie
Wang, Zheng
Fan, Jia
Dai, Zhi
He, Yi-Feng
Qiu, Shuang-Jian
Huang, Xiao-Wu
Sun, Jian
Xiao, Yong-Sheng
Song, Kang
Shi, Ying-Hong
Sun, Qi-Man
Yang, Xin-Rong
Shi, Guo-Ming
Yu, Lei
Yang, Guo-Huan
Ding, Zhen-Bin
Gao, Qiang
Tang, Zhao-You
Zhou, Jian
author_sort Hu, Jie
collection PubMed
description BACKGROUND: Recurrence prediction of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) patients undergoing liver transplantation (LT) present a great challenge because of a lack of biomarkers. Genetic variations play an important role in tumor development and metastasis. METHODS: Oligonucleotide microarrays were used to evaluate the genetic characteristics of tumor DNA in 30 HBV-related HCC patients who were underwent LT. Recurrence-related single-nucleotide polymorphism were selected, and their prognostic value was assessed and validated in two independent cohorts of HCC patients (N = 102 and N = 77), using pretransplant plasma circulating DNA. Prognostic significance was assessed by Kaplan-Meier survival estimates and log-rank tests. Multivariate analyses were performed to evaluate prognosis-related factors. RESULTS: rs894151 and rs12438080 were significantly associated with recurrence (P = .003 and P = .004, respectively). Multivariate analyses demonstrated that the co-index of the 2 SNPs was an independent prognostic factor for recurrence (P = .040). Similar results were obtained in the third cohort (N = 77). Furthermore, for HCC patients (all the 3 cohorts) exceeding Milan criteria, the co-index was a prognostic factor for recurrence and survival (P<.001 and P = .002, respectively). CONCLUSIONS: Our study demonstrated first that genetic variations of rs894151 and rs12438080 in pretransplant plasma circulating DNA are promising prognostic markers for tumor recurrence in HCC patients undergoing LT and identify a subgroup of patients who, despite having HCC exceeding Milan criteria, have a low risk of post-transplant recurrence.
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spelling pubmed-31878412011-10-13 Genetic Variations in Plasma Circulating DNA of HBV-Related Hepatocellular Carcinoma Patients Predict Recurrence after Liver Transplantation Hu, Jie Wang, Zheng Fan, Jia Dai, Zhi He, Yi-Feng Qiu, Shuang-Jian Huang, Xiao-Wu Sun, Jian Xiao, Yong-Sheng Song, Kang Shi, Ying-Hong Sun, Qi-Man Yang, Xin-Rong Shi, Guo-Ming Yu, Lei Yang, Guo-Huan Ding, Zhen-Bin Gao, Qiang Tang, Zhao-You Zhou, Jian PLoS One Research Article BACKGROUND: Recurrence prediction of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) patients undergoing liver transplantation (LT) present a great challenge because of a lack of biomarkers. Genetic variations play an important role in tumor development and metastasis. METHODS: Oligonucleotide microarrays were used to evaluate the genetic characteristics of tumor DNA in 30 HBV-related HCC patients who were underwent LT. Recurrence-related single-nucleotide polymorphism were selected, and their prognostic value was assessed and validated in two independent cohorts of HCC patients (N = 102 and N = 77), using pretransplant plasma circulating DNA. Prognostic significance was assessed by Kaplan-Meier survival estimates and log-rank tests. Multivariate analyses were performed to evaluate prognosis-related factors. RESULTS: rs894151 and rs12438080 were significantly associated with recurrence (P = .003 and P = .004, respectively). Multivariate analyses demonstrated that the co-index of the 2 SNPs was an independent prognostic factor for recurrence (P = .040). Similar results were obtained in the third cohort (N = 77). Furthermore, for HCC patients (all the 3 cohorts) exceeding Milan criteria, the co-index was a prognostic factor for recurrence and survival (P<.001 and P = .002, respectively). CONCLUSIONS: Our study demonstrated first that genetic variations of rs894151 and rs12438080 in pretransplant plasma circulating DNA are promising prognostic markers for tumor recurrence in HCC patients undergoing LT and identify a subgroup of patients who, despite having HCC exceeding Milan criteria, have a low risk of post-transplant recurrence. Public Library of Science 2011-10-05 /pmc/articles/PMC3187841/ /pubmed/21998744 http://dx.doi.org/10.1371/journal.pone.0026003 Text en Hu et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Hu, Jie
Wang, Zheng
Fan, Jia
Dai, Zhi
He, Yi-Feng
Qiu, Shuang-Jian
Huang, Xiao-Wu
Sun, Jian
Xiao, Yong-Sheng
Song, Kang
Shi, Ying-Hong
Sun, Qi-Man
Yang, Xin-Rong
Shi, Guo-Ming
Yu, Lei
Yang, Guo-Huan
Ding, Zhen-Bin
Gao, Qiang
Tang, Zhao-You
Zhou, Jian
Genetic Variations in Plasma Circulating DNA of HBV-Related Hepatocellular Carcinoma Patients Predict Recurrence after Liver Transplantation
title Genetic Variations in Plasma Circulating DNA of HBV-Related Hepatocellular Carcinoma Patients Predict Recurrence after Liver Transplantation
title_full Genetic Variations in Plasma Circulating DNA of HBV-Related Hepatocellular Carcinoma Patients Predict Recurrence after Liver Transplantation
title_fullStr Genetic Variations in Plasma Circulating DNA of HBV-Related Hepatocellular Carcinoma Patients Predict Recurrence after Liver Transplantation
title_full_unstemmed Genetic Variations in Plasma Circulating DNA of HBV-Related Hepatocellular Carcinoma Patients Predict Recurrence after Liver Transplantation
title_short Genetic Variations in Plasma Circulating DNA of HBV-Related Hepatocellular Carcinoma Patients Predict Recurrence after Liver Transplantation
title_sort genetic variations in plasma circulating dna of hbv-related hepatocellular carcinoma patients predict recurrence after liver transplantation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3187841/
https://www.ncbi.nlm.nih.gov/pubmed/21998744
http://dx.doi.org/10.1371/journal.pone.0026003
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