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Genetic Variations in Plasma Circulating DNA of HBV-Related Hepatocellular Carcinoma Patients Predict Recurrence after Liver Transplantation
BACKGROUND: Recurrence prediction of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) patients undergoing liver transplantation (LT) present a great challenge because of a lack of biomarkers. Genetic variations play an important role in tumor development and metastasis. METHODS: Oligon...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3187841/ https://www.ncbi.nlm.nih.gov/pubmed/21998744 http://dx.doi.org/10.1371/journal.pone.0026003 |
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author | Hu, Jie Wang, Zheng Fan, Jia Dai, Zhi He, Yi-Feng Qiu, Shuang-Jian Huang, Xiao-Wu Sun, Jian Xiao, Yong-Sheng Song, Kang Shi, Ying-Hong Sun, Qi-Man Yang, Xin-Rong Shi, Guo-Ming Yu, Lei Yang, Guo-Huan Ding, Zhen-Bin Gao, Qiang Tang, Zhao-You Zhou, Jian |
author_facet | Hu, Jie Wang, Zheng Fan, Jia Dai, Zhi He, Yi-Feng Qiu, Shuang-Jian Huang, Xiao-Wu Sun, Jian Xiao, Yong-Sheng Song, Kang Shi, Ying-Hong Sun, Qi-Man Yang, Xin-Rong Shi, Guo-Ming Yu, Lei Yang, Guo-Huan Ding, Zhen-Bin Gao, Qiang Tang, Zhao-You Zhou, Jian |
author_sort | Hu, Jie |
collection | PubMed |
description | BACKGROUND: Recurrence prediction of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) patients undergoing liver transplantation (LT) present a great challenge because of a lack of biomarkers. Genetic variations play an important role in tumor development and metastasis. METHODS: Oligonucleotide microarrays were used to evaluate the genetic characteristics of tumor DNA in 30 HBV-related HCC patients who were underwent LT. Recurrence-related single-nucleotide polymorphism were selected, and their prognostic value was assessed and validated in two independent cohorts of HCC patients (N = 102 and N = 77), using pretransplant plasma circulating DNA. Prognostic significance was assessed by Kaplan-Meier survival estimates and log-rank tests. Multivariate analyses were performed to evaluate prognosis-related factors. RESULTS: rs894151 and rs12438080 were significantly associated with recurrence (P = .003 and P = .004, respectively). Multivariate analyses demonstrated that the co-index of the 2 SNPs was an independent prognostic factor for recurrence (P = .040). Similar results were obtained in the third cohort (N = 77). Furthermore, for HCC patients (all the 3 cohorts) exceeding Milan criteria, the co-index was a prognostic factor for recurrence and survival (P<.001 and P = .002, respectively). CONCLUSIONS: Our study demonstrated first that genetic variations of rs894151 and rs12438080 in pretransplant plasma circulating DNA are promising prognostic markers for tumor recurrence in HCC patients undergoing LT and identify a subgroup of patients who, despite having HCC exceeding Milan criteria, have a low risk of post-transplant recurrence. |
format | Online Article Text |
id | pubmed-3187841 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-31878412011-10-13 Genetic Variations in Plasma Circulating DNA of HBV-Related Hepatocellular Carcinoma Patients Predict Recurrence after Liver Transplantation Hu, Jie Wang, Zheng Fan, Jia Dai, Zhi He, Yi-Feng Qiu, Shuang-Jian Huang, Xiao-Wu Sun, Jian Xiao, Yong-Sheng Song, Kang Shi, Ying-Hong Sun, Qi-Man Yang, Xin-Rong Shi, Guo-Ming Yu, Lei Yang, Guo-Huan Ding, Zhen-Bin Gao, Qiang Tang, Zhao-You Zhou, Jian PLoS One Research Article BACKGROUND: Recurrence prediction of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC) patients undergoing liver transplantation (LT) present a great challenge because of a lack of biomarkers. Genetic variations play an important role in tumor development and metastasis. METHODS: Oligonucleotide microarrays were used to evaluate the genetic characteristics of tumor DNA in 30 HBV-related HCC patients who were underwent LT. Recurrence-related single-nucleotide polymorphism were selected, and their prognostic value was assessed and validated in two independent cohorts of HCC patients (N = 102 and N = 77), using pretransplant plasma circulating DNA. Prognostic significance was assessed by Kaplan-Meier survival estimates and log-rank tests. Multivariate analyses were performed to evaluate prognosis-related factors. RESULTS: rs894151 and rs12438080 were significantly associated with recurrence (P = .003 and P = .004, respectively). Multivariate analyses demonstrated that the co-index of the 2 SNPs was an independent prognostic factor for recurrence (P = .040). Similar results were obtained in the third cohort (N = 77). Furthermore, for HCC patients (all the 3 cohorts) exceeding Milan criteria, the co-index was a prognostic factor for recurrence and survival (P<.001 and P = .002, respectively). CONCLUSIONS: Our study demonstrated first that genetic variations of rs894151 and rs12438080 in pretransplant plasma circulating DNA are promising prognostic markers for tumor recurrence in HCC patients undergoing LT and identify a subgroup of patients who, despite having HCC exceeding Milan criteria, have a low risk of post-transplant recurrence. Public Library of Science 2011-10-05 /pmc/articles/PMC3187841/ /pubmed/21998744 http://dx.doi.org/10.1371/journal.pone.0026003 Text en Hu et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Hu, Jie Wang, Zheng Fan, Jia Dai, Zhi He, Yi-Feng Qiu, Shuang-Jian Huang, Xiao-Wu Sun, Jian Xiao, Yong-Sheng Song, Kang Shi, Ying-Hong Sun, Qi-Man Yang, Xin-Rong Shi, Guo-Ming Yu, Lei Yang, Guo-Huan Ding, Zhen-Bin Gao, Qiang Tang, Zhao-You Zhou, Jian Genetic Variations in Plasma Circulating DNA of HBV-Related Hepatocellular Carcinoma Patients Predict Recurrence after Liver Transplantation |
title | Genetic Variations in Plasma Circulating DNA of HBV-Related Hepatocellular Carcinoma Patients Predict Recurrence after Liver Transplantation |
title_full | Genetic Variations in Plasma Circulating DNA of HBV-Related Hepatocellular Carcinoma Patients Predict Recurrence after Liver Transplantation |
title_fullStr | Genetic Variations in Plasma Circulating DNA of HBV-Related Hepatocellular Carcinoma Patients Predict Recurrence after Liver Transplantation |
title_full_unstemmed | Genetic Variations in Plasma Circulating DNA of HBV-Related Hepatocellular Carcinoma Patients Predict Recurrence after Liver Transplantation |
title_short | Genetic Variations in Plasma Circulating DNA of HBV-Related Hepatocellular Carcinoma Patients Predict Recurrence after Liver Transplantation |
title_sort | genetic variations in plasma circulating dna of hbv-related hepatocellular carcinoma patients predict recurrence after liver transplantation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3187841/ https://www.ncbi.nlm.nih.gov/pubmed/21998744 http://dx.doi.org/10.1371/journal.pone.0026003 |
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