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Analyzing and minimizing PCR amplification bias in Illumina sequencing libraries
Despite the ever-increasing output of Illumina sequencing data, loci with extreme base compositions are often under-represented or absent. To evaluate sources of base-composition bias, we traced genomic sequences ranging from 6% to 90% GC through the process by quantitative PCR. We identified PCR du...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3188800/ https://www.ncbi.nlm.nih.gov/pubmed/21338519 http://dx.doi.org/10.1186/gb-2011-12-2-r18 |