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Sequential expression of putative stem cell markers in gastric carcinogenesis
BACKGROUND: Gastric carcinogenesis has been well documented in the step-wise histopathological model, known as Correa pathway. Several biomarkers including CD44, Musashi-1 and CD133 have been reported as putative stem cell (PSC) markers. METHODS: We investigated expression of PSC markers CD44, Musas...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3188930/ https://www.ncbi.nlm.nih.gov/pubmed/21829201 http://dx.doi.org/10.1038/bjc.2011.287 |
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author | Wang, T Ong, C W Shi, J Srivastava, S Yan, B Cheng, C L Yong, W P Chan, S L Yeoh, K G Iacopetta, B Salto-Tellez, M |
author_facet | Wang, T Ong, C W Shi, J Srivastava, S Yan, B Cheng, C L Yong, W P Chan, S L Yeoh, K G Iacopetta, B Salto-Tellez, M |
author_sort | Wang, T |
collection | PubMed |
description | BACKGROUND: Gastric carcinogenesis has been well documented in the step-wise histopathological model, known as Correa pathway. Several biomarkers including CD44, Musashi-1 and CD133 have been reported as putative stem cell (PSC) markers. METHODS: We investigated expression of PSC markers CD44, Musashi-1 and CD133 in relation to gastric carcinogenesis and prognosis and chemoresponse. Immunohistochemistry staining was performed in gastric cancer (GC) clinical specimens representing different steps of the Correa pathway. Gastric cancer samples taken before and after neoadjuvant chemotherapy with docetaxel, cisplatin and capecitabine (DCX) were also evaluated for PSC marker expression. RESULTS: We showed that the expression of three PSC markers was significantly elevated in GC relative to normal gastric mucosa (P<0.001 for each marker). Precancerous lesions, including intestinal metaplasia and dysplasia, demonstrated increased expression of CD44 and Musashi-1. CD133 was predominantly expressed along the border between intramucosal carcinoma and connective tissue at later stages. High CD44 and CD133 expression showed prognostic value for worse patient survival (P=0.014 and P=0.019, respectively). A small number of tumours with high expression of CD44 and CD133 showed pathological response to DCX-based neoadjuvant chemotherapy. CONCLUSION: CD44 and Musashi-1 are frequently expressed in both premalignant gastric lesions and invasive GC, whereas CD133 expression is restricted mainly to neoplastic tissues. |
format | Online Article Text |
id | pubmed-3188930 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-31889302012-08-23 Sequential expression of putative stem cell markers in gastric carcinogenesis Wang, T Ong, C W Shi, J Srivastava, S Yan, B Cheng, C L Yong, W P Chan, S L Yeoh, K G Iacopetta, B Salto-Tellez, M Br J Cancer Molecular Diagnostics BACKGROUND: Gastric carcinogenesis has been well documented in the step-wise histopathological model, known as Correa pathway. Several biomarkers including CD44, Musashi-1 and CD133 have been reported as putative stem cell (PSC) markers. METHODS: We investigated expression of PSC markers CD44, Musashi-1 and CD133 in relation to gastric carcinogenesis and prognosis and chemoresponse. Immunohistochemistry staining was performed in gastric cancer (GC) clinical specimens representing different steps of the Correa pathway. Gastric cancer samples taken before and after neoadjuvant chemotherapy with docetaxel, cisplatin and capecitabine (DCX) were also evaluated for PSC marker expression. RESULTS: We showed that the expression of three PSC markers was significantly elevated in GC relative to normal gastric mucosa (P<0.001 for each marker). Precancerous lesions, including intestinal metaplasia and dysplasia, demonstrated increased expression of CD44 and Musashi-1. CD133 was predominantly expressed along the border between intramucosal carcinoma and connective tissue at later stages. High CD44 and CD133 expression showed prognostic value for worse patient survival (P=0.014 and P=0.019, respectively). A small number of tumours with high expression of CD44 and CD133 showed pathological response to DCX-based neoadjuvant chemotherapy. CONCLUSION: CD44 and Musashi-1 are frequently expressed in both premalignant gastric lesions and invasive GC, whereas CD133 expression is restricted mainly to neoplastic tissues. Nature Publishing Group 2011-08-23 2011-08-09 /pmc/articles/PMC3188930/ /pubmed/21829201 http://dx.doi.org/10.1038/bjc.2011.287 Text en Copyright © 2011 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Molecular Diagnostics Wang, T Ong, C W Shi, J Srivastava, S Yan, B Cheng, C L Yong, W P Chan, S L Yeoh, K G Iacopetta, B Salto-Tellez, M Sequential expression of putative stem cell markers in gastric carcinogenesis |
title | Sequential expression of putative stem cell markers in gastric carcinogenesis |
title_full | Sequential expression of putative stem cell markers in gastric carcinogenesis |
title_fullStr | Sequential expression of putative stem cell markers in gastric carcinogenesis |
title_full_unstemmed | Sequential expression of putative stem cell markers in gastric carcinogenesis |
title_short | Sequential expression of putative stem cell markers in gastric carcinogenesis |
title_sort | sequential expression of putative stem cell markers in gastric carcinogenesis |
topic | Molecular Diagnostics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3188930/ https://www.ncbi.nlm.nih.gov/pubmed/21829201 http://dx.doi.org/10.1038/bjc.2011.287 |
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