Cargando…
The Complete Genome Sequence of Thermoproteus tenax: A Physiologically Versatile Member of the Crenarchaeota
Here, we report on the complete genome sequence of the hyperthermophilic Crenarchaeum Thermoproteus tenax (strain Kra1, DSM 2078(T)) a type strain of the crenarchaeotal order Thermoproteales. Its circular 1.84-megabase genome harbors no extrachromosomal elements and 2,051 open reading frames are ide...
Autores principales: | , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3189178/ https://www.ncbi.nlm.nih.gov/pubmed/22003381 http://dx.doi.org/10.1371/journal.pone.0024222 |
_version_ | 1782213442630844416 |
---|---|
author | Siebers, Bettina Zaparty, Melanie Raddatz, Guenter Tjaden, Britta Albers, Sonja-Verena Bell, Steve D. Blombach, Fabian Kletzin, Arnulf Kyrpides, Nikos Lanz, Christa Plagens, André Rampp, Markus Rosinus, Andrea von Jan, Mathias Makarova, Kira S. Klenk, Hans-Peter Schuster, Stephan C. Hensel, Reinhard |
author_facet | Siebers, Bettina Zaparty, Melanie Raddatz, Guenter Tjaden, Britta Albers, Sonja-Verena Bell, Steve D. Blombach, Fabian Kletzin, Arnulf Kyrpides, Nikos Lanz, Christa Plagens, André Rampp, Markus Rosinus, Andrea von Jan, Mathias Makarova, Kira S. Klenk, Hans-Peter Schuster, Stephan C. Hensel, Reinhard |
author_sort | Siebers, Bettina |
collection | PubMed |
description | Here, we report on the complete genome sequence of the hyperthermophilic Crenarchaeum Thermoproteus tenax (strain Kra1, DSM 2078(T)) a type strain of the crenarchaeotal order Thermoproteales. Its circular 1.84-megabase genome harbors no extrachromosomal elements and 2,051 open reading frames are identified, covering 90.6% of the complete sequence, which represents a high coding density. Derived from the gene content, T. tenax is a representative member of the Crenarchaeota. The organism is strictly anaerobic and sulfur-dependent with optimal growth at 86°C and pH 5.6. One particular feature is the great metabolic versatility, which is not accompanied by a distinct increase of genome size or information density as compared to other Crenarchaeota. T. tenax is able to grow chemolithoautotrophically (CO(2)/H(2)) as well as chemoorganoheterotrophically in presence of various organic substrates. All pathways for synthesizing the 20 proteinogenic amino acids are present. In addition, two presumably complete gene sets for NADH:quinone oxidoreductase (complex I) were identified in the genome and there is evidence that either NADH or reduced ferredoxin might serve as electron donor. Beside the typical archaeal A(0)A(1)-ATP synthase, a membrane-bound pyrophosphatase is found, which might contribute to energy conservation. Surprisingly, all genes required for dissimilatory sulfate reduction are present, which is confirmed by growth experiments. Mentionable is furthermore, the presence of two proteins (ParA family ATPase, actin-like protein) that might be involved in cell division in Thermoproteales, where the ESCRT system is absent, and of genes involved in genetic competence (DprA, ComF) that is so far unique within Archaea. |
format | Online Article Text |
id | pubmed-3189178 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-31891782011-10-14 The Complete Genome Sequence of Thermoproteus tenax: A Physiologically Versatile Member of the Crenarchaeota Siebers, Bettina Zaparty, Melanie Raddatz, Guenter Tjaden, Britta Albers, Sonja-Verena Bell, Steve D. Blombach, Fabian Kletzin, Arnulf Kyrpides, Nikos Lanz, Christa Plagens, André Rampp, Markus Rosinus, Andrea von Jan, Mathias Makarova, Kira S. Klenk, Hans-Peter Schuster, Stephan C. Hensel, Reinhard PLoS One Research Article Here, we report on the complete genome sequence of the hyperthermophilic Crenarchaeum Thermoproteus tenax (strain Kra1, DSM 2078(T)) a type strain of the crenarchaeotal order Thermoproteales. Its circular 1.84-megabase genome harbors no extrachromosomal elements and 2,051 open reading frames are identified, covering 90.6% of the complete sequence, which represents a high coding density. Derived from the gene content, T. tenax is a representative member of the Crenarchaeota. The organism is strictly anaerobic and sulfur-dependent with optimal growth at 86°C and pH 5.6. One particular feature is the great metabolic versatility, which is not accompanied by a distinct increase of genome size or information density as compared to other Crenarchaeota. T. tenax is able to grow chemolithoautotrophically (CO(2)/H(2)) as well as chemoorganoheterotrophically in presence of various organic substrates. All pathways for synthesizing the 20 proteinogenic amino acids are present. In addition, two presumably complete gene sets for NADH:quinone oxidoreductase (complex I) were identified in the genome and there is evidence that either NADH or reduced ferredoxin might serve as electron donor. Beside the typical archaeal A(0)A(1)-ATP synthase, a membrane-bound pyrophosphatase is found, which might contribute to energy conservation. Surprisingly, all genes required for dissimilatory sulfate reduction are present, which is confirmed by growth experiments. Mentionable is furthermore, the presence of two proteins (ParA family ATPase, actin-like protein) that might be involved in cell division in Thermoproteales, where the ESCRT system is absent, and of genes involved in genetic competence (DprA, ComF) that is so far unique within Archaea. Public Library of Science 2011-10-07 /pmc/articles/PMC3189178/ /pubmed/22003381 http://dx.doi.org/10.1371/journal.pone.0024222 Text en This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. |
spellingShingle | Research Article Siebers, Bettina Zaparty, Melanie Raddatz, Guenter Tjaden, Britta Albers, Sonja-Verena Bell, Steve D. Blombach, Fabian Kletzin, Arnulf Kyrpides, Nikos Lanz, Christa Plagens, André Rampp, Markus Rosinus, Andrea von Jan, Mathias Makarova, Kira S. Klenk, Hans-Peter Schuster, Stephan C. Hensel, Reinhard The Complete Genome Sequence of Thermoproteus tenax: A Physiologically Versatile Member of the Crenarchaeota |
title | The Complete Genome Sequence of Thermoproteus tenax: A Physiologically Versatile Member of the Crenarchaeota
|
title_full | The Complete Genome Sequence of Thermoproteus tenax: A Physiologically Versatile Member of the Crenarchaeota
|
title_fullStr | The Complete Genome Sequence of Thermoproteus tenax: A Physiologically Versatile Member of the Crenarchaeota
|
title_full_unstemmed | The Complete Genome Sequence of Thermoproteus tenax: A Physiologically Versatile Member of the Crenarchaeota
|
title_short | The Complete Genome Sequence of Thermoproteus tenax: A Physiologically Versatile Member of the Crenarchaeota
|
title_sort | complete genome sequence of thermoproteus tenax: a physiologically versatile member of the crenarchaeota |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3189178/ https://www.ncbi.nlm.nih.gov/pubmed/22003381 http://dx.doi.org/10.1371/journal.pone.0024222 |
work_keys_str_mv | AT siebersbettina thecompletegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT zapartymelanie thecompletegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT raddatzguenter thecompletegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT tjadenbritta thecompletegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT alberssonjaverena thecompletegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT bellsteved thecompletegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT blombachfabian thecompletegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT kletzinarnulf thecompletegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT kyrpidesnikos thecompletegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT lanzchrista thecompletegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT plagensandre thecompletegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT ramppmarkus thecompletegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT rosinusandrea thecompletegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT vonjanmathias thecompletegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT makarovakiras thecompletegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT klenkhanspeter thecompletegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT schusterstephanc thecompletegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT henselreinhard thecompletegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT siebersbettina completegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT zapartymelanie completegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT raddatzguenter completegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT tjadenbritta completegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT alberssonjaverena completegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT bellsteved completegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT blombachfabian completegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT kletzinarnulf completegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT kyrpidesnikos completegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT lanzchrista completegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT plagensandre completegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT ramppmarkus completegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT rosinusandrea completegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT vonjanmathias completegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT makarovakiras completegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT klenkhanspeter completegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT schusterstephanc completegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota AT henselreinhard completegenomesequenceofthermoproteustenaxaphysiologicallyversatilememberofthecrenarchaeota |