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TILLING - a shortcut in functional genomics
Recent advances in large-scale genome sequencing projects have opened up new possibilities for the application of conventional mutation techniques in not only forward but also reverse genetics strategies. TILLING (Targeting Induced Local Lesions IN Genomes) was developed a decade ago as an alternati...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer-Verlag
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3189332/ https://www.ncbi.nlm.nih.gov/pubmed/21912935 http://dx.doi.org/10.1007/s13353-011-0061-1 |
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author | Kurowska, Marzena Daszkowska-Golec, Agata Gruszka, Damian Marzec, Marek Szurman, Miriam Szarejko, Iwona Maluszynski, Miroslaw |
author_facet | Kurowska, Marzena Daszkowska-Golec, Agata Gruszka, Damian Marzec, Marek Szurman, Miriam Szarejko, Iwona Maluszynski, Miroslaw |
author_sort | Kurowska, Marzena |
collection | PubMed |
description | Recent advances in large-scale genome sequencing projects have opened up new possibilities for the application of conventional mutation techniques in not only forward but also reverse genetics strategies. TILLING (Targeting Induced Local Lesions IN Genomes) was developed a decade ago as an alternative to insertional mutagenesis. It takes advantage of classical mutagenesis, sequence availability and high-throughput screening for nucleotide polymorphisms in a targeted sequence. The main advantage of TILLING as a reverse genetics strategy is that it can be applied to any species, regardless of its genome size and ploidy level. The TILLING protocol provides a high frequency of point mutations distributed randomly in the genome. The great mutagenic potential of chemical agents to generate a high rate of nucleotide substitutions has been proven by the high density of mutations reported for TILLING populations in various plant species. For most of them, the analysis of several genes revealed 1 mutation/200–500 kb screened and much higher densities were observed for polyploid species, such as wheat. High-throughput TILLING permits the rapid and low-cost discovery of new alleles that are induced in plants. Several research centres have established a TILLING public service for various plant species. The recent trends in TILLING procedures rely on the diversification of bioinformatic tools, new methods of mutation detection, including mismatch-specific and sensitive endonucleases, but also various alternatives for LI-COR screening and single nucleotide polymorphism (SNP) discovery using next-generation sequencing technologies. The TILLING strategy has found numerous applications in functional genomics. Additionally, wide applications of this throughput method in basic and applied research have already been implemented through modifications of the original TILLING strategy, such as Ecotilling or Deletion TILLING. |
format | Online Article Text |
id | pubmed-3189332 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Springer-Verlag |
record_format | MEDLINE/PubMed |
spelling | pubmed-31893322011-10-12 TILLING - a shortcut in functional genomics Kurowska, Marzena Daszkowska-Golec, Agata Gruszka, Damian Marzec, Marek Szurman, Miriam Szarejko, Iwona Maluszynski, Miroslaw J Appl Genet Plant Genetics • Review Recent advances in large-scale genome sequencing projects have opened up new possibilities for the application of conventional mutation techniques in not only forward but also reverse genetics strategies. TILLING (Targeting Induced Local Lesions IN Genomes) was developed a decade ago as an alternative to insertional mutagenesis. It takes advantage of classical mutagenesis, sequence availability and high-throughput screening for nucleotide polymorphisms in a targeted sequence. The main advantage of TILLING as a reverse genetics strategy is that it can be applied to any species, regardless of its genome size and ploidy level. The TILLING protocol provides a high frequency of point mutations distributed randomly in the genome. The great mutagenic potential of chemical agents to generate a high rate of nucleotide substitutions has been proven by the high density of mutations reported for TILLING populations in various plant species. For most of them, the analysis of several genes revealed 1 mutation/200–500 kb screened and much higher densities were observed for polyploid species, such as wheat. High-throughput TILLING permits the rapid and low-cost discovery of new alleles that are induced in plants. Several research centres have established a TILLING public service for various plant species. The recent trends in TILLING procedures rely on the diversification of bioinformatic tools, new methods of mutation detection, including mismatch-specific and sensitive endonucleases, but also various alternatives for LI-COR screening and single nucleotide polymorphism (SNP) discovery using next-generation sequencing technologies. The TILLING strategy has found numerous applications in functional genomics. Additionally, wide applications of this throughput method in basic and applied research have already been implemented through modifications of the original TILLING strategy, such as Ecotilling or Deletion TILLING. Springer-Verlag 2011-09-13 2011 /pmc/articles/PMC3189332/ /pubmed/21912935 http://dx.doi.org/10.1007/s13353-011-0061-1 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Plant Genetics • Review Kurowska, Marzena Daszkowska-Golec, Agata Gruszka, Damian Marzec, Marek Szurman, Miriam Szarejko, Iwona Maluszynski, Miroslaw TILLING - a shortcut in functional genomics |
title | TILLING - a shortcut in functional genomics |
title_full | TILLING - a shortcut in functional genomics |
title_fullStr | TILLING - a shortcut in functional genomics |
title_full_unstemmed | TILLING - a shortcut in functional genomics |
title_short | TILLING - a shortcut in functional genomics |
title_sort | tilling - a shortcut in functional genomics |
topic | Plant Genetics • Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3189332/ https://www.ncbi.nlm.nih.gov/pubmed/21912935 http://dx.doi.org/10.1007/s13353-011-0061-1 |
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