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Comparative study of fungal cell disruption—scope and limitations of the methods

Simple and effective protocols of cell wall disruption were elaborated for tested fungal strains: Penicillium citrinum, Aspergillus fumigatus, Rhodotorula gracilis. Several techniques of cell wall disintegration were studied, including ultrasound disintegration, homogenization in bead mill, applicat...

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Autores principales: Klimek-Ochab, Magdalena, Brzezińska-Rodak, Małgorzata, Żymańczyk-Duda, Ewa, Lejczak, Barbara, Kafarski, Paweł
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3189342/
https://www.ncbi.nlm.nih.gov/pubmed/21901292
http://dx.doi.org/10.1007/s12223-011-0069-2
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author Klimek-Ochab, Magdalena
Brzezińska-Rodak, Małgorzata
Żymańczyk-Duda, Ewa
Lejczak, Barbara
Kafarski, Paweł
author_facet Klimek-Ochab, Magdalena
Brzezińska-Rodak, Małgorzata
Żymańczyk-Duda, Ewa
Lejczak, Barbara
Kafarski, Paweł
author_sort Klimek-Ochab, Magdalena
collection PubMed
description Simple and effective protocols of cell wall disruption were elaborated for tested fungal strains: Penicillium citrinum, Aspergillus fumigatus, Rhodotorula gracilis. Several techniques of cell wall disintegration were studied, including ultrasound disintegration, homogenization in bead mill, application of chemicals of various types, and osmotic shock. The release of proteins from fungal cells and the activity of a cytosolic enzyme, glucose-6-phosphate dehydrogenase, in the crude extracts were assayed to determine and compare the efficacy of each method. The presented studies allowed adjusting the particular method to a particular strain. The mechanical methods of disintegration appeared to be the most effective for the disintegration of yeast, R. gracilis, and filamentous fungi, A. fumigatus and P. citrinum. Ultrasonication and bead milling led to obtaining fungal cell-free extracts containing high concentrations of soluble proteins and active glucose-6-phosphate dehydrogenase systems.
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spelling pubmed-31893422011-10-12 Comparative study of fungal cell disruption—scope and limitations of the methods Klimek-Ochab, Magdalena Brzezińska-Rodak, Małgorzata Żymańczyk-Duda, Ewa Lejczak, Barbara Kafarski, Paweł Folia Microbiol (Praha) Article Simple and effective protocols of cell wall disruption were elaborated for tested fungal strains: Penicillium citrinum, Aspergillus fumigatus, Rhodotorula gracilis. Several techniques of cell wall disintegration were studied, including ultrasound disintegration, homogenization in bead mill, application of chemicals of various types, and osmotic shock. The release of proteins from fungal cells and the activity of a cytosolic enzyme, glucose-6-phosphate dehydrogenase, in the crude extracts were assayed to determine and compare the efficacy of each method. The presented studies allowed adjusting the particular method to a particular strain. The mechanical methods of disintegration appeared to be the most effective for the disintegration of yeast, R. gracilis, and filamentous fungi, A. fumigatus and P. citrinum. Ultrasonication and bead milling led to obtaining fungal cell-free extracts containing high concentrations of soluble proteins and active glucose-6-phosphate dehydrogenase systems. Springer Netherlands 2011-09-08 2011 /pmc/articles/PMC3189342/ /pubmed/21901292 http://dx.doi.org/10.1007/s12223-011-0069-2 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Article
Klimek-Ochab, Magdalena
Brzezińska-Rodak, Małgorzata
Żymańczyk-Duda, Ewa
Lejczak, Barbara
Kafarski, Paweł
Comparative study of fungal cell disruption—scope and limitations of the methods
title Comparative study of fungal cell disruption—scope and limitations of the methods
title_full Comparative study of fungal cell disruption—scope and limitations of the methods
title_fullStr Comparative study of fungal cell disruption—scope and limitations of the methods
title_full_unstemmed Comparative study of fungal cell disruption—scope and limitations of the methods
title_short Comparative study of fungal cell disruption—scope and limitations of the methods
title_sort comparative study of fungal cell disruption—scope and limitations of the methods
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3189342/
https://www.ncbi.nlm.nih.gov/pubmed/21901292
http://dx.doi.org/10.1007/s12223-011-0069-2
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